| TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤 |
| |
| 引用本文： | 杨崛圣,樊虎熊,胡姗,高日峰,卢飞,王恒,唐燕华.TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤[J].基础医学与临床,2020,40(2):209-214. |
| |
| 作者姓名： | 杨崛圣 樊虎熊 胡姗 高日峰 卢飞 王恒 唐燕华 |
| |
| 作者单位： | 南昌大学第二附属医院心脏大血管外科,江西 南昌330031;南昌大学第二附属医院南昌大学研究学院医学部,江西 南昌330031;南昌大学第二附属医院麻醉科,江西 南昌330031;南昌大学第二附属医院 介入室,江西 南昌330031;平顶山市第二人民医院 胸心外科,河南 平顶山467000 |
| |
| 摘 要： | 目的研究瞬时受体电位通道7(TRPM7)干扰载体对低氧大鼠心肌细胞系(H9C2)修复功能的影响及其机理。方法建立H9C2心肌细胞低氧/复氧模型,构建TRPM7干扰表达载体转染H9C2细胞,用RT-qPCR与Western blot检测H9C2细胞低氧诱导因子(HIF1-α)和TRPM7的表达,流式细胞计量术检测胞内钙离子水平(Ca^2+]i)和细胞凋亡,确定TRPM7对H9C2心肌细胞的修复作用。结果H9C2细胞转染TRPM7干扰载体后,细胞的HIF1-α和TRPM7表达显著下降(P<0.05),Ca^2+]i降低及凋亡率减少(P<0.05)。结论TRPM7干扰载体可能通过PI3K/Akt通路对低氧H9C2心肌细胞有显著的修复作用。
|
| 关 键 词： | TRPM7干扰载体 [CA^2+]I 细胞凋亡 |
TRPM7 interference vector repaires injury caused by hypoxia/reoxygenation in cardiomyocyte cell line H9C2 |
| |
| YANG Jue-sheng,FAN Hu-xiong,HU Shan,GAO Ri-feng,LU Fei,WANG Heng,TANG Yan-hua.TRPM7 interference vector repaires injury caused by hypoxia/reoxygenation in cardiomyocyte cell line H9C2[J].Basic Medical Sciences and Clinics,2020,40(2):209-214. |
| |
| Authors: | YANG Jue-sheng FAN Hu-xiong HU Shan GAO Ri-feng LU Fei WANG Heng TANG Yan-hua |
| |
| Institution: | (Department of Cardiovascular Surgery;Department of Anesthesiology,the Second Affiliated Hospital of Nanchang University;Medical Department of Graduate School of Nanchang University;Department of Interventional,the Second Affiliated Hospital of Nanchang University,Nanchang 330031;Department of Cardiothoracic Surgery,Pingdingshan Second People's Hospital,Pingdingshan 467000,China) |
| |
| Abstract: | Objective To study the repair function and mechanism of TRPM7 interference vector in hypoxic myocardial cell line(H9C2).Methods The hypoxia/reoxygenation model of H9C2 cardiomyocytes was established and the TRPM7 interference expression vector was constructed to transfect H9C2 cells.The expression of HIF1-αand TRPM7 in H9C2 cells was detected by RT-qPCR and Western blot.Ca^2+]i and apoptosis was determined by flow cytometry.Results When H9C2 cells were transfected with TRPM7 interference vector,the expression of HIF1-αTTRPM7,theCa^2+]i concentration and the apoptosis rate all decreased.Conclusions TRPM7 interference vector has significant repairing effect on hypoxia H9C2 cardiomyocytes,and the mechanism may be mediated PI3K/Akt pathway. |
| |
| Keywords: | TRPM7 interference vector [Ca^2+]i cell apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
