骨髓和脂肪来源的间充质干细胞免疫调节作用的比较

目的比较脂肪源间充质干细胞(AMSCs)和骨髓来源间充质干细胞(BMSCs)的免疫调节能力的差异。方法用流式细胞仪分析AMSCs和BMSCs的表型。通过MSCs和淋巴细胞共培养体系,检测MSCs对T细胞增殖、周期、活化、凋亡以及Th细胞分化的影响。结果表型分析结果显示AMSCs和BMSCs的表型基本一致。AMSCs和BMSCs都能抑制T细胞的增殖和早期活化,并在调节辅助性T细胞亚群的分化上作用类似。但在MSC对活化后T细胞的凋亡影响方面,BMSCs能够抑制活化T细胞的凋亡,而AMSC没有这种作用。结论 AMSCs和BMSCs对T淋巴细胞的影响基本类似,其对活化T细胞凋亡影响的差异还有待进一步的机制研究。

Comparison of immuno-modulatory properties between bone-marrow derived MSCs and adipose derived MSCs

Objective To compare MSCs’ immuno-modulatory property between bone-marrow derived and adipose derived MSCs.Methods Phenotype analysis of both MSCs populations was detected by flow-cytometry.To analyse immuno-modulatory effect of MSCs,proliferation,cell cycle,activation,apoptosis and Th1/Th2 balance were analyzed after co-culturing lymphocytes and MSCs.Results Phenotype analysis showed that AMSCs had the similar expression of markers with BMSCs.Both AMSCs and BMSCs could inhibit T cells proliferation and activation.Also MSCs’ effect on Th cell differentiation into Th1 or Th2 was similar between AMSCs and BMSCs.Nevertheless,BMSCs could suppress activated T cell apoptosis,while AMSCs could not.Conclusions AMSCs and BMSCs showed similar immuno-modulatroy effect on T lymphocytes,however the difference effect on activated T cell apoptosis of the two cell populations is still unclear and the mechanism needs to explore.