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| 细胞毒T淋巴细胞相关抗原4基因多态性与吸毒并HIV感染相关 | |
| 引用本文： | 刘莹,刁波,申元英,周芳,冯勇,罗凡,侯炜.细胞毒T淋巴细胞相关抗原4基因多态性与吸毒并HIV感染相关[J].基础医学与临床,2011,31(7). |
| 作者姓名： | 刘莹 刁波 申元英 周芳 冯勇 罗凡 侯炜 |
| 作者单位： | 1. 武汉大学,病毒学国家重点实验室,医学病毒学研究所,湖北,武汉,430071 2. 大理学院,公共卫生学院,云南,大理,67100 3. 广州军区武汉总医院,医学实验科,湖北,武汉,430070 |
| 基金项目： | 国家自然科学基金(30972754); 中央高校基本科研业务费专项资金资助 |
| 摘 要： | 目的研究细胞毒T淋巴细胞相关抗原4(CTLA-4)基因外显子1的49位点A/G和启动子-318位点C/T多态性与吸毒并HIV感染的相关性。方法 采用序列特异性引物聚合酶链反应(PCR-SSP)方法,检测24例单纯吸毒者、41例吸毒合并HIV感染患者以及204例正常对照者CTLA-4基因外显子1的49位点A/G和启动子-318位点C/T的基因型。结果 与正常对照组比较,吸毒组C/C(12.50%vs51.47%)(P<0.01)型频率明显下降;在吸毒合并HIV感染患者组,CTLA-4A+49G基因型差异无统计学意义(P>0.05);CTLA-4C-318T基因型差异有统计学意义(P<0.01),C/T(68.3%vs45.6%)、T/T(21.95%vs2.94%)型频率明显上升,C/C(9.76%vs51.47%)型频率显著下降。与吸毒组比较,吸毒合并HIV感染组中CTLA-4A+49G及CTLA-4C-318T各基因型频率变化无统计学差异(P>0.05)。结论 吸毒合并HIV感染与CTLA-4基因动态密切相关,C-318T基因型C/T、T/T型与吸毒合并HIV感染呈正相关,C/C行呈负相关。 |
| 关 键 词： | 吸毒并HIV感染 细胞毒T淋巴细胞相关抗原4 基因多态性 基因型 |
Relationship between cytotoxic T lymphocyte-associated antigen 4 gene haplotype and drug use accompanied with HIV infection |
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| LIU Ying,DIAO Bo,SHEN Yuan-ying,ZHOU Fang,FENG Yong,LUO Fan,HOU Wei.Relationship between cytotoxic T lymphocyte-associated antigen 4 gene haplotype and drug use accompanied with HIV infection[J].Basic Medical Sciences and Clinics,2011,31(7). | |
| Authors: | LIU Ying DIAO Bo SHEN Yuan-ying ZHOU Fang FENG Yong LUO Fan HOU Wei |
| Institution: | LIU Ying1,DIAO Bo1,SHEN Yuan-ying2,ZHOU Fang3,FENG Yong1,LUO Fan1,HOU Wei1(1.State Key Laboratory of Virology/Institute of Virology,School of Medicine,Wuhan University,Wuhan 430071,2.College of Public Hygieneof Dali University,Dali 67100,3.Dept.of Medicine Experiment.Wuhan General Hospital Guangzhou Military Region,Wuhan 430070 China) |
| Abstract: | Objective To investigate the association of gene polymorphism of cytotoxic T lymphocyte-associated antigen 4(CTLA-4) with drug users with HIV infection.Methods The A+49G transition polymorphism at position 49(exon 1) and C-318T transition polymorphism at position 318 in promoter of the CTLA-4 gene were determined by polymerase chain reaction with sequence specific primers(PCR-SSP) method in 24 drug users,41 drug users with HIV Infection patients and 204 healthy controls.Results Compared with control group,i... |
| Keywords: | drug users with HIV infection cytotoxic T lymphocyte-associated antigen gene polymorphism genotype |
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