| Ghrelin对糖尿病大鼠海马神经元凋亡及其认知功能的影响 |
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| 引用本文： | 马楼艳,张栋珉,张颖,高原,柴李殷,赵柯湘,夏丽,肖谦.Ghrelin对糖尿病大鼠海马神经元凋亡及其认知功能的影响[J].基础医学与临床,2011,31(6). |
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| 作者姓名： | 马楼艳 张栋珉 张颖 高原 柴李殷 赵柯湘 夏丽 肖谦 |
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| 作者单位： | 重庆医科大学,附属第一医院,老年科,内分泌组,重庆,400016 |
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| 基金项目： | 重庆市卫生局科技重点项目(2010-1-8); 重庆市自然科学基金(CSTC,2010BB5396); 重庆医科大学资助项目(xbyb2007018) |
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| 摘 要： | 目的观察Ghrelin对糖尿病大鼠海马神经元凋亡及其认知功能的影响。方法 40只雄性SD大鼠随机均分为对照组、糖尿病组、糖尿病+Ghrelin组和糖尿病+Ghrelin+D-lys3-GHRP-6组,每组10只。建立STZ糖尿病模型,open-field实验排除合并抑郁症的糖尿病大鼠,Morris水迷宫测试学习与记忆能力,RT-PCR检测海马caspase-3mRNA的表达,免疫组化检测海马神经元caspase-3和BCL-xl蛋白表达,原位末端标记法检测海马神经元的凋亡。结果与对照组大鼠相比,糖尿病组和糖尿病+Ghrelin+D-lys3-GHRP-6组学习与记忆能力显著受损(P<0.05),海马部位的caspase-3 mRNA及蛋白表达明显升高(P<0.05),BCL-xl蛋白表达显著下降(P<0.05),海马神经元的凋亡增加了49%(P<0.05)。糖尿病+Ghrelin组学习和记忆成绩明显优于糖尿病组(P<0.05),其海马部位的caspase-3 mRNA及蛋白表达显著下降(P<0.05),BCL-xl蛋白表达明显升高(P<0.05),海马神经元的凋亡减少了42%。结论 Ghrelin对糖尿病大鼠...
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| 关 键 词： | Ghrelin 糖尿病脑病 凋亡 认知功能 |
Effects of Ghrelin on the hippocampus neuronal apoptosis and the cognitive function of diabetic rats |
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| MA Lou-yan,ZHANG Dong-min,ZHANG Yin,GAO Yuan,CHAI Li-yin,ZHAO Ke-xiang,XIA Li,XIAO Qian.Effects of Ghrelin on the hippocampus neuronal apoptosis and the cognitive function of diabetic rats[J].Basic Medical Sciences and Clinics,2011,31(6). |
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| Authors: | MA Lou-yan ZHANG Dong-min ZHANG Yin GAO Yuan CHAI Li-yin ZHAO Ke-xiang XIA Li XIAO Qian |
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| Institution: | MA Lou-yan,ZHANG Dong-min,ZHANG Yin,GAO Yuan,CHAI Li-yin,ZHAO Ke-xiang,XIA Li,XIAO Qian(Endocrinology Group,Dept.of Geriatrics,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To explore the effects of Ghrelin on the hippocampus neuronal apoptosis and the cognitive function of diabetic rats.Methods Forty SD male rats were meanly divided into normal group,diabetic modle group,Ghrelin treatment group,Ghrelin and D-lys-3-GHRP-6 treatment gooup randomly.Streptozotocin-diabetic rats model were established,diabetic rats with depression were screened by open-field test,learning and memory behaviors were measured using a spatial version of the Morris water maze test.The mRNA le... |
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| Keywords: | Ghrelin diabetic encephalopathy apoptosis cognition |
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