喹啉酸致脑损伤大鼠中枢与外周胆碱能受体活性的变化

采用大鼠脑室内微量反奥秒主射喹啉酸（ｑｕｉｎｏｌｉｎｉｅ ａｃｉｄ，ＱＡ）造成的学习记忆障碍模型和自然衰老大鼠，测定其学习记忆功能和脑内胆碱能神经系统的变化。结果显示：ＱＡ模型的学习记忆能力明显受损，脑内胆碱能神经标志ｈＡＴ活必和，脑海马、大脑皮层胆碱能毒蕈碱受体（ＭＲ）结合活性分别降低３０％和２６％。烟碱受人本（ＮＲ）结合活性分别降低３３％和２７％，并较老年鼠明显，进一步发现，各组外周脾淋巴细胞

Changes of cholinergic receptor activities in central and peripheral system after quinolinic acid (QA) caused cerebral injury in rats

We used the learning and memory impairment model established through intraventricular injection of QArepeatedly, to observe the functional changes of central cholinergic nerve and their reflects on the peripheral lymphaticsystem . There were a significant decline of cholineacetyltransferase (ChAT) in cerebral cortex and hippocampus afterQA injection. We also found the lower binding activities of M-and N-cholinergic receptors in hippocampus and cortexin QA group significantly than in the PBS group (P<0.01). Furthermore, the binding activities of M-and N-receptorsof spleen lymphocytes as well as those in brain were improved in each group. The results suggest that certain lesionof the centhel nerve system function can be reflected through peripheral biochemical parameter, which is an importantreference to diagnose the changes of the central nervous system.