| 移植转染ADM或HGF基因的骨髓间充质干细胞治疗大鼠心肌梗死 |
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| 引用本文： | 苏津自,林茂海,林美萍.移植转染ADM或HGF基因的骨髓间充质干细胞治疗大鼠心肌梗死[J].基础医学与临床,2010,30(2):125-132. |
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| 作者姓名： | 苏津自 林茂海 林美萍 |
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| 作者单位： | 1. 福建医科大学附属第一医院,心内科
2. 福建省高血压研究所,福建,福州,350005 |
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| 基金项目： | 福建省自然科学基金(C061001) |
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| 摘 要： | 目的探讨移植转染两种不同基因的骨髓间充质干细胞移植治疗大鼠心肌梗死的作用。方法分离、扩增培养大鼠骨髓间充质干细胞;ELISA检测细胞上清液ADM或HGF的含量;制备大鼠心肌梗死模型,心肌局部注射移植经DAPI标记的MSCs;多普勒超声检测大鼠心功能;荧光显微镜观察细胞存活及分布;免疫组化检测新生血管密度及移植细胞在心梗区的分化。结果转染Ad-ADM或Ad-HGF后,MSCs可有效表达ADM或HGF;与单纯MSCs组相比,两基因组细胞均表达TNI,均有connexin 43的表达增加(P<0.05),心梗区新生血管密度增高(P<0.01),左室射血分数(EF)增加(P<0.01);两组间各指标无差别。结论不同基因修饰的MSCs移植均可增强单纯MSCs对心肌梗死大鼠的治疗作用。
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| 关 键 词： | 肾上腺髓质素 肝细胞生长因子 骨髓间充质干细胞 心肌梗死 细胞移植 |
| 收稿时间： | 2009-2-13 |
| 修稿时间： | 2009-6-2 |
Effects of transplantation of mesenchymal stem cells combining with ADM or HGF gene in the treatment of myocardial infarction in rats |
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| SU Jin-zi,UN Mao-hai,UN Mei-ping.Effects of transplantation of mesenchymal stem cells combining with ADM or HGF gene in the treatment of myocardial infarction in rats[J].Basic Medical Sciences and Clinics,2010,30(2):125-132. |
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| Authors: | SU Jin-zi UN Mao-hai UN Mei-ping |
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| Institution: | 1.Department of Cardiology;the First Affiliated Hospital of Fujian Medical University;2.Fujian Institute of HypertensionResearch;Fuzhou 350005;China |
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| Abstract: | Objective To investigate effects of transplantation of mesenchymal stem cells combining with two different gene transfection on the treatment of myocardial infarction in rats.Methods MSCs were isolated and expanded using the preplating method.Ad-ADM or Ad-HGF expression in MSCs and its secretion in culture medium were measured by ELISA.The left anterior descending branch of rats was ligated to establish a myocardial infarction model.Then the pretreated MSCs were labeled by DAPI,through injection directly in... |
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| Keywords: | adrenomedullin hepatocyte growth factor mesenchymal stem cells myocardial infarction cell transplantation |
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