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| 赖氨大黄酸通过激活JNK诱导宫颈癌HeLa细胞凋亡 | |
| 引用本文： | 甄永占,林雅军,骆广玲,赵毓芳,高俊玲.赖氨大黄酸通过激活JNK诱导宫颈癌HeLa细胞凋亡[J].基础医学与临床,2011,31(1). |
| 作者姓名： | 甄永占 林雅军 骆广玲 赵毓芳 高俊玲 |
| 作者单位： | 1. 华北煤炭医学院,基础部,组织学与胚胎学教研室,河北,唐山,063000 2. 卫生部北京医院,卫生部北京老年医学研究所,北京,100730 3. 开滦医疗集团林西医院妇产科,河北,唐山,063000 |
| 摘 要： | 目的研究赖氨大黄酸(RHL)对人宫颈癌HeLa细胞增殖、凋亡的影响,并探讨其作用机制。方法用MTT法检测细胞增殖;用流式细胞仪检测细胞凋亡,用Western blot检测凋亡相关蛋白及JNK蛋白与蛋白磷酸化水平。结果 RHL能有效抑制宫颈癌HeLa细胞增殖,并能诱导其凋亡,随药物浓度的增加,细胞凋亡率也逐渐升高;RHL能够激活caspase-3、caspase-7和PARP,并激活磷酸化的JNK表达,磷酸化的JNK表达增加在RHL的诱导凋亡中起主要作用。结论 RHL通过激活JNK-caspase-PARP信号通路抑制HeLa细胞增殖,并诱导其凋亡,赖氨大黄酸解决了大黄酸不溶于水的问题,有望成为临床肿瘤辅助化疗药物。 |
| 关 键 词： | 赖氨大黄酸 细胞凋亡 宫颈癌 JNK |
Rhein lysinate induces apoptosis of human cervical cancer HeLa cells by activating c-Jun N-terminal kinase(JNK) |
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| ZHEN Yong-zhan,LIN Ya-jun,LUO Guang-ling,ZHAO Yu-fang,GAO Jun-ling.Rhein lysinate induces apoptosis of human cervical cancer HeLa cells by activating c-Jun N-terminal kinase(JNK)[J].Basic Medical Sciences and Clinics,2011,31(1). | |
| Authors: | ZHEN Yong-zhan LIN Ya-jun LUO Guang-ling ZHAO Yu-fang GAO Jun-ling |
| Institution: | ZHEN Yong-zhan1,LIN Ya-jun2,LUO Guang-ling3,ZHAO Yu-fang1,GAO Jun-ling1(1.Dept.of Histology and Embryology,North China Coal Medical Univesrity,Tangshan 063000,2.the Key Laboratory of Geriatrics,Beijing Hospital & Beijing Institute of Geriatrics,Ministry of Health,Beijing 100730,3.Dept.of Gynaecology and Obstetrics,Linxi Hospital,Kailuan Medical Systems,China) |
| Abstract: | Objective To investigate the effect of rhein lysinate(RHL) on inducing human cervical cancer cell line HeLa apoptosis and the role of activation of c-Jun N-terminal kinase(JNK) in cell apoptosis.MethodsMTT assay was used to detect HeLa cell proliferation.Cell apoptosis was analyzed by flow cytometry.The protein associated with apoptosis and the protein phosphorylation of JNK was detected by Western blot.Results RHL remarkably inhibited cervical cancer HeLa cells proliferation.The IC50 value for 48 and 72 h ... |
| Keywords: | rhein lysinate cervical cancer apoptosis JNK |
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