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长链非编码RNA AK046999在小鼠大脑皮质发育中的表达及功能
引用本文:李嘉宁,周俊洁,刘伟,彭小忠,舒鹏程.长链非编码RNA AK046999在小鼠大脑皮质发育中的表达及功能[J].基础医学与临床,2017,37(11).
作者姓名:李嘉宁  周俊洁  刘伟  彭小忠  舒鹏程
作者单位:中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室生物化学与分子生物学系, 医学灵长类研究中心, 神经科学中心,北京100005
基金项目:国家重点研发计划,重点基础研究发展计划,国家自然科学基金,中国医学科学院创新基金
摘    要:目的检测长链非编码RNA AK046999在小鼠大脑皮质发育过程中的表达及作用。方法 1)通过UCSC基因组浏览器获取其基因组座位,利用Coding Potential Calculator工具对其编码能力进行预测;2)原位杂交及免疫荧光技术确定其在大脑皮质发育过程中的表达谱;3)TALEN技术构建AK046999完全敲除小鼠,并且在DNA及RNA水平对其进行敲除鉴定;4)免疫荧光及TUNEL技术对敲除小鼠大脑皮质在发育过程的功能进行检测。结果 1)AK046999可认为是非编码RNA(Coding Potential Score-1);2)AK046999在小鼠大脑皮质发育过程中,前体区相对高表达;3)成功构建了AK046999敲除小鼠;4)与对照相比,AK046999敲除小鼠中表达PAX6、TBR2和NEUROD2的细胞数量无明显改变,并且在这两组小鼠中凋亡细胞的数量无明显改变。结论 AK046999在发育期的小鼠大脑皮质表达,但敲除后不影响小鼠大脑皮质中神经祖细胞的增殖、分化和细胞凋亡过程。

关 键 词:AK046999  长链非编码RNA  时空表达谱  大脑皮质发育

Expression and function of long non-coding RNA AK046999 in the development of mouse cerebral cortex
LI Jia-ning,ZHOU Jun-jie,LIU Wei,PENG Xiao-zhong,SHU Peng-cheng.Expression and function of long non-coding RNA AK046999 in the development of mouse cerebral cortex[J].Basic Medical Sciences and Clinics,2017,37(11).
Authors:LI Jia-ning  ZHOU Jun-jie  LIU Wei  PENG Xiao-zhong  SHU Peng-cheng
Abstract:Objective To detect the expression and the role of the long non -coding RNA AK046999 in the development of mouse cerebral cortex.Methods 1) The AK046999 locus was obtained from the UCSC genome browser ,and the coding ability of protein was predicted by Coding Potential Calculator ; 2) The expression profile of AK046999 in the development of mouse cerebral cortex was analyzed by immunofluorescence and in situ hybridization;3)The AK046999 knockout mouse was constructed by TALEN technique and identified at the level of DNA and RNA; 4)The phenotype of knockout mouse cerebral cortex was analyzed by immunofluorescence and TUNEL assay.Results 1)AK046999 had weak coding ability and could be considered as non -coding RNA; 2)AK046999 was highly expressed in VZ /SVZ of the development of mouse cerebral cortex; 3)The AK046999 knockout mouse was successfully constructed; 4) Immunofluorescence results showed that there were no obvious changes in the markers of PAX6, TBR2 and NEUROD2 compared with the control, and apoptotic cells also had no obvious change.Conclusions The AK046999 is expressed in the cerebral cortex of developing mouse and AK046999 knockout has no effect on proliferation, differentiation and apoptosis of neural progenitor cells in the cerebral cortex .
Keywords:AK046999  long non-coding RNA  spatio-temporal expression  cerebral cortex development
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