| 骨形态发生蛋白9抑制人乳腺癌MDA-MB-231细胞体外侵袭和迁移 |
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| 引用本文： | 王科,冯红蕾,孙笑笑,罗进勇,张彦.骨形态发生蛋白9抑制人乳腺癌MDA-MB-231细胞体外侵袭和迁移[J].基础医学与临床,2011,31(4). |
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| 作者姓名： | 王科 冯红蕾 孙笑笑 罗进勇 张彦 |
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| 作者单位： | 重庆医科大学临床检验诊断学教育部重点实验室,重庆,400016 |
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| 摘 要： | 目的 探讨骨形态发生蛋白9(BMP9)对乳腺癌MDA-MB-231细胞侵袭、迁移能力的影响.方法 RT-PCR法检测MDA-MB-231细胞系中BMP9的表达;扩增高滴度的BMP9表达腺病毒,感染MDA-MB-231细胞,制备高表达BMP9的重组MDA-MB-231/BMP9细胞,以此作为实验组;同时以含GFP的窄载腺病毒感染该细胞为MDA-MB-231/GFP,联合空白MDA-MB-231共同作为对照组;通过平板集落形成实验、细胞划痕实验、Transwell侵袭实验检测重组MDA-MB-231/BMP9细胞侵袭及迁移能力.结果 与对照组细胞相比,实验组细胞中BMP9 mRNA表达水平明显增高;实验组细胞集落形成率由对照的90.6%±2.5%与85.6%±3.5%显著下降至57.3%±4.5%(P＜0.05);实验组划痕愈合率由对照的95.4%±3.1%与92.5%±2.4%下降至74.0%±3.6%(P＜0.05);实验组穿膜细胞数由对照的(255.3±16.1)个与(267.3±14.9)个下降至(150.3±14.6)个(P＜0.05).结论 BMP9可以在体外抑制乳腺癌MDA-MB-231细胞的侵袭与迁移.
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| 关 键 词： | 骨形态发生蛋白9 乳腺癌 肿瘤侵袭 |
BMP9 inhibits invasion and migration of human MDA-MB-231 cells in vitro |
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| WANG Ke,FENG Hong-lei,SUN Xiao-xiao,LUO Jin-yong,ZHANG Yan.BMP9 inhibits invasion and migration of human MDA-MB-231 cells in vitro[J].Basic Medical Sciences and Clinics,2011,31(4). |
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| Authors: | WANG Ke FENG Hong-lei SUN Xiao-xiao LUO Jin-yong ZHANG Yan |
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| Institution: | WANG Ke,FENG Hong-lei,SUN Xiao-xiao,LUO Jin-yong,ZHANG Yan*(The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the correlation among bone morphogenetic protein 9(BMP9) expression,migration and invasion of breast cancer MDA-MB-231 cells in vitro.Methods The expression of BMP9 in MDA-MB-231 was detected by RT-PCR,high titer of adenovirus vector expressing BMP9 was used to infect MDA-MB-231.Experimental group: MDA-MB-231/BMP9,control group: MDA-MB-231/GFP and MDA-MB-231.To detect the changes of invasion and migration in the recombinant MDA-MB-231/BMP9 cells colony-forming assay,cell wounding as... |
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| Keywords: | BMP9 breast cancer tumor invasion |
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