Splenocyte response to T cell-derived cytokines in thymectomized Xenopus. |
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Authors: | Sarah L Turner Trudy L Horton Pamela Ritchie and John D Horton |
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Institution: | Department of Biological Sciences, University of Durham, South Road, Durham DH1 3LE, U.K. |
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Abstract: | A miniaturized, “hanging-drop” bioassay reveals that splenocytes from earlythymectomized (Tx) Xenopus can respond (by enhanced thymidine incorporation) to thymicdependent “cytokines” generated in PHA- or alloantigen-stimulated cultures. Preliminary evidence, using fluorescence activated cell sorting, indicates that surface IgM− splenocytes, rather than sIgM+ cells, from Tx toads are sensitive to the crude, splenocyte-derived, active supernatants. Although these responsive cells display residual, but low, reactivity to PHA, their thymus independence is suggested by flow cytometric observations using the anti-T cell monoclonal antibody XT-1. The development of “T-like” cells in Tx Xenopus is discussed. |
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Keywords: | Xenopus Thymectomy Cytokine bioassays Mixed leucocyte culture T-cell mitogen Flow cytometry sIg+ & sIg− cells |
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