| 北京市2007-2010年风疹病原学监测 |
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| 引用本文： | 张铁钢,杨洁,陈萌,龚成,罗明,吴疆,黄迎春.北京市2007-2010年风疹病原学监测[J].中华微生物学和免疫学杂志,2011,31(5). |
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| 作者姓名： | 张铁钢 杨洁 陈萌 龚成 罗明 吴疆 黄迎春 |
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| 作者单位： | 1. 北京市疾病预防控制中心免疫预防所,100013
2. 北京联合大学生物化学工程学院,100023 |
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| 摘 要： | 目的 对北京地区风疹病原学进行监测.方法 收集风疹疑似病例血清标本和尿液和(或)咽拭子,用于血清学检测和病毒分离.病毒分离物和临床标本中的核酸利用real-time PCR方法进行检测.结果 2007-2010年,共收集99个风疹疑似病例血清标本和临床标本,血清学确诊55个病例(56%),病毒分离确诊51个病例(52%).回顾性检测疑似风疹病例及尿液和(或)咽拭子核酸,共确诊72个风疹病例(73%).分离的风疹病毒基因型主要为1E基因型.结论 北京地区主要流行的风疹病毒为1E基因型别.Abstract：Objective To clarify the pathogen for rubella in Beijing from 2007 to 2010. Methods Beijing Center for Disease Preventipn and Control ( CDC ) collected the specimens (including blood, urine and throat swab specimens) frqm clinically diagnosed rubella cases for serological test and virus isolation. The nucleic acid of rubella virus in clinical specimens and isolations was detected by real-time PCR. Results Fifty-five out of 99 blood specimens were positive for anti- rubella IgM. Fifty-one out of 99 clinically diagnosed rubella cases were confirmed as rubella cases by virus isolation. Seventy-two were confirmed as rubella virus infections with real-time PCR method for detecting the nucleic acid of rubella virus in clinical specimens. Compared with the sequences of reference strains of rubella virus, all of detected rubella virus belonged the IE gene type. Conclusion This study indicates that IE gene type virus was the predominant endemic rubella virus in Beijing.
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| 关 键 词： | 风疹病毒 基因型 |
The pathogen surveillance of rubella virus in Beijing from 2007 to 2010 |
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| ZHANG Tie-gang,YANG Jie,CHEN Meng,GONG Cheng,LUO Ming,WU Jiang,HUANG Ying-chun.The pathogen surveillance of rubella virus in Beijing from 2007 to 2010[J].Chinese Journal of Microbiology and Immunology,2011,31(5). |
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| Authors: | ZHANG Tie-gang YANG Jie CHEN Meng GONG Cheng LUO Ming WU Jiang HUANG Ying-chun |
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| Abstract: | Objective To clarify the pathogen for rubella in Beijing from 2007 to 2010. Methods Beijing Center for Disease Preventipn and Control ( CDC ) collected the specimens (including blood, urine and throat swab specimens) frqm clinically diagnosed rubella cases for serological test and virus isolation. The nucleic acid of rubella virus in clinical specimens and isolations was detected by real-time PCR. Results Fifty-five out of 99 blood specimens were positive for anti- rubella IgM. Fifty-one out of 99 clinically diagnosed rubella cases were confirmed as rubella cases by virus isolation. Seventy-two were confirmed as rubella virus infections with real-time PCR method for detecting the nucleic acid of rubella virus in clinical specimens. Compared with the sequences of reference strains of rubella virus, all of detected rubella virus belonged the IE gene type. Conclusion This study indicates that IE gene type virus was the predominant endemic rubella virus in Beijing. |
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| Keywords: | Real-time PCR |
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