首页 | 本学科首页   官方微博 | 高级检索  
     

用CL法和微量MPO法观察人参皂甙对人PMN吞噬杀菌作用
引用本文:张力平,沈海中,程松高. 用CL法和微量MPO法观察人参皂甙对人PMN吞噬杀菌作用[J]. 中华微生物学和免疫学杂志, 1994, 0(2)
作者姓名:张力平  沈海中  程松高
作者单位:首都医科大学微生物学教研室
基金项目:中国科学院自然科学基金
摘    要:采用化学发光(CL)法和髓过氧化物酶(MPO)微量测定法检测了人参茎叶皂甙对人多形核白细胞(PMN)吞噬金黄色葡萄球菌的影响。实验结果表明,人参茎叶皂甙作用过的PMN-CL反应(CL30和Peak)和MPO释放水平均增强,对CL30与CFU、MPO与CFU行直线相关分析均呈密切相关(r=0.958,p<0.001;r=0.875,p<0.01)。本实验提示,PMN-CL法和MPO微量测定法是研究药物对PMN吞噬杀菌功能影响的客观、定量和快速的方法。

关 键 词:多形核白细胞,化学发光,髓过氧化物酶,人参茎叶皂甙

INVESTIGATION OF GINSENOSIDE ON HUMAN PMN PHAGOCYTOSIS BY CL AND MPO MICROMEASUREMENT
Zhang Li-ping,Shen Hai-zhong and Cheng Son-gao. INVESTIGATION OF GINSENOSIDE ON HUMAN PMN PHAGOCYTOSIS BY CL AND MPO MICROMEASUREMENT[J]. Chinese Journal of Microbiology and Immunology, 1994, 0(2)
Authors:Zhang Li-ping  Shen Hai-zhong  Cheng Son-gao
Abstract:The effect of ginsenoside on human polymorphonuclear leukocytte(PMN)phagocytosis and killing function wasevaluated by chemiluminescence(CL ) assay and myeloperoxidase(MPO )release micromeasurement. The resultsshowed that the CL response(CL30 and Peak )and MPO release level of ginsenoside-pretreated PMN were en-hanced.The CL30 was closely related to CFU of remainder S.aureus(r=0.875,P<0.01),the MPO release levelwas also closely related to CFU r=0.958,P<0.001),The experiment indicated that the CL assay and MPO re-lease micromeasurement were the quantitative ,objective and rapid method for evaluating the phagocytosis andkilling function of PMN effected by the medicines .
Keywords:Polymorphonuclear leukocyte(PMN)  Chemiluminescence(CL)  Myeloperoxidase(MPO)  Ginsenoside  
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号