| 依地酸与环丙沙星联合应用对豚鼠铜绿假单胞菌生物被膜感染模型的作用 |
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| 引用本文： | 林雅茵,余加林,芦起,林丽华.依地酸与环丙沙星联合应用对豚鼠铜绿假单胞菌生物被膜感染模型的作用[J].中华微生物学和免疫学杂志,2011,31(10):912-915. |
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| 作者姓名： | 林雅茵 余加林 芦起 林丽华 |
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| 作者单位： | 400014 重庆医科大学附属儿童医院新生儿中心,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地 |
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| 摘 要： | 目的 探讨依地酸(ethylenediamine tetraacetic acid,EDTA)与环丙沙星联用对豚鼠体内铜绿假单胞菌生物被膜感染的清除作用.方法 吸入法建立豚鼠肺部铜绿假单胞菌生物被膜感染模型；EDTA腹腔注射、环丙沙星雾化吸入,单独及联合作用于感染后豚鼠；平板计数法对比药物作用前后生物被膜菌落数变化,H...
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| 关 键 词： | 铜绿假单胞菌 生物被膜 依地酸钠 |
Effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm infection in guineapig |
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| LIN Ya-yin,YU Jia-lin,LU Qi,LIN Li-hua.Effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm infection in guineapig[J].Chinese Journal of Microbiology and Immunology,2011,31(10):912-915. |
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| Authors: | LIN Ya-yin YU Jia-lin LU Qi LIN Li-hua |
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| Abstract: | Objective To investigate the effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm in vivo.Methods Pseudomonas aeruginosa was inhaled into the lung of guinea pigs and colonized,formed biofilm.After 7 days,the model was treated with ciprofloxacin,EDTA alone,or a combination of both for 7 days.The number of colony in the lungs is measured by agar plate.The pathological change of the lung is observed by hematoxylin and eosin (HE) staining and scanning electron microscope.Results EDTA combined with ciprofloxacin make the number of bacteria in the lungs reduced from l05 CFU/g to 10 CFU/g(t =24.67,P＜0.05),the lung lesion was less-sever histophathologically.Conclusion The combination of EDTA with ciprofloxacin has significant activity to remove mucoid PA biofilm in vivo. |
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| Keywords: | Pseudomonas aeruginosa Biofilm Ethylenediamine tetraacetic acid |
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