恶性肿瘤胸腹水中TIL生物学活性研究

从卵巢癌腹水和肺癌胸水中分离出肿瘤浸润淋巴细胞（ＴＩＬ），用ｒＩＬ－２诱导培养，观察其增殖活性。同时观察了ＰＪ－ＣＷ、ＰＯＧＰ、ＰＨＡ等对其杀伤活性的调节作用。结果表明，在培养５～１０天时ＴＩＬ增殖较快，其表面产生的ＣＤ３、ＣＤ４、ＣＤ８、Ｔａｃ等抗原，随培养时间的延长，表达量增加，尤以ＣＤ８增加较明显，ＣＤ４／ＣＤ８比值逐降。其表面产生的ＨＬＡ－ＤＲ、ＨＬＡ－ＡＢＣ抗原，在培养１５天内有增加表现，以后下降。其杀伤活性在培养５～１０天较高，其后活性下降。在培养系统中加入ＰＪ－ＣＷ、ＰＯＧＰ、ＰＨＡ等后，培养５天时，ＴＩＬ杀伤活性分别为８２．７０％，８５．５０％，８４．３５％（对照组为内保持较高的杀伤活性。

Study on biological activity of TIL from malignant pleursl effusion and ascites and the regulation of its killing activity

Tumor infiltration lymphocytes (TIL) were separated from ovary cancer ascites and lung cancer pleural effusion , and cultured with rlL -2. We observed the dynamics of its proliferation activity , production of surface antigens and killing activity ,in addition ,the regulation effect of PJ-CW ,POGP ,PHA on the killing activity of TIL we were also observed. It showed iT IL proliferated fast at culture for 5-10 days , with prolongation of the culture time ,production of CD3 , 4 , 8 ,Tac antigen ,especially CD8 increased ,so the ratio of CD4 /CD8 decreased gradually. The production of HLA -DR , HLA-ABC antigens increased within culture for 15 days , and then decreased . The killing activity increased and reached the top at culture for 5 days ,and then decreased. Adding PJ-CW ,POGP and PHA into the culture medium , the killing activity of TIL at culture for 5-10 days was 82.70% , 85.50% and 84. 35% respectively ,higher than that of control (66. 80%) obviously, and maintained higher killing activity within 20 days.