| 痢疾菌侵袭性质粒抗原（Ipa）及O抗原与侵入细胞能力的关系研究 |
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| 引用本文： | 章容萍,陈恩临.痢疾菌侵袭性质粒抗原（Ipa）及O抗原与侵入细胞能力的关系研究[J].中华微生物学和免疫学杂志,1994(1). |
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| 作者姓名： | 章容萍 陈恩临 |
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| 作者单位： | 天津医学院微生物学教研室，天津第二医学院微生物学教研室 |
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| 摘 要： | 采用痢疾菌Ｉｐａ＋Ｏａｇ－，ＩｐａＯａｇ＋及Ｉｐａ＋Ｏａｇ＋等三种不同的菌株分别进行了毒力及毒力相关表型实验。结果发现，Ｉｐａ＋ＯａＧ－株Ｓ１Ｒ１０１／ｐＨＳ４１０８与Ｉｐａ＋Ｏａｇ＋株ＢＳ１６９／ｐＨＳ４１０８都有侵入ＨｅＬａ细胞的能力，而Ｉｐａ－Ｏａｇ＋株Ｔ３２则无侵入ＨｅＬａ细胞的能力，只表达多肽ａ、ｅ、ｄ的菌株Ｓ１Ｒ１０２／ｐＪＭ５６亦不能侵入ＨｅＬａ细胞。从而证明，侵袭性质粒抗原（Ｉｐａ）对于细菌侵入ＨｅＬａ细胞则是足够的，其中多肽ｂ起关键性作用，而Ｏ抗原并不参与侵入过程或不成为其必要的前提条件。
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| 关 键 词： | 痢疾菌 侵袭性外膜蛋白 O抗原 毒力表型 |
THE PATHOGENESIS OF SHIGELLASPECIES:ROLES OF INVASIVE OUTER MEMBRANE
PROTEINS AND O ANTIGEN IN THE ENTRY PROCESS |
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| Zhang Rong -ping,Chen
En-lin.THE PATHOGENESIS OF SHIGELLASPECIES:ROLES OF INVASIVE OUTER MEMBRANE
PROTEINS AND O ANTIGEN IN THE ENTRY PROCESS[J].Chinese Journal of Microbiology and Immunology,1994(1). |
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| Authors: | Zhang Rong -ping Chen
En-lin |
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| Abstract: | he virulence and
the virulence-associated phenotypes of Shigella species were investigated with Ipa Oag-,Ipa
Oag ,and Ipa-Oag strains,respectively.Our results showed that strains
S1R101/pHS4108(Ip8 Oag-)andBS169/pHS4108(Ipa Oag )were capable of entering HeLa cells
;while S. sonnei strain S1R102/pJM56 whichcould only express a,c,and d and S.flexneri strain
T32(Ipa-Oag )were incapable of entering HeLa Cells.Wesuggest hereby that invasive plasmid
polypeptides alone may be sufficient for the bacillus into the cells,and O antigen may not be
involved or play little role,if any ,in this process. |
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| Keywords: | Shigella species Invasion outer membrane protein O antigen Virulence phenotype |
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