Protective effect of two new nanovaccines against Pseudomonas aeruginosa based on LPS and OPS: A comparison study |
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| Affiliation: | 1. Dept. of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran;2. Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran;1. Department of Ophthalmology, Federal University of São Paulo, Botucatu Street, 806, Vila Clementino, 04023-062 São Paulo, SP, Brazil;2. Regional Integrated University Alto Uruguay and Missions, Sete de Setembro Avenue, 1621, Erechim, Rio Grande do Sul 99709-910, Brazil;3. Integrated Research Group in Biomarkers, René Rachou Institute, Oswaldo Cruz Foundation-FIOCRUZ, Augusto de Lima Avenue, 1715, Belo Horizonte, Minas Gerais 30190-002, Brazil;4. Federal University of Uberlândia, Pará Avenue, 1720, Bairro Umuarama, 38405-320 Uberlândia, Minas Gerais, Brazil;5. Protozoology Laboratory, Preventive Veterinary Medicine Department, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, 86.057-970 Londrina, PR, Brazil;6. Clinica Silveira, Sete de Setembro Avenue, 1502, 99700-003 Erechim, Rio Grande do Sul, Brazil;1. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;2. Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;3. Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia;4. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;1. Weifang People’s Hospital, 261000 Weifang, China;2. School of Basic Medicine, Weifang Medical University, 261053 Weifang, China;3. Department of Pharmacology, School of Medicine, Southeast University, 210009 Nanjing, China;1. Pharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Centre of PLA General Hospital, Beijing, China;2. Medical School of Chinese PLA, Beijing, China;3. Chinese People''s Armed Police Force Hospital of Beijing, Beijing, China;1. Cancer Immunology Project (CIP), Universal Scientific Education and Research Network (USERN), Tehran, Iran;2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;3. School of Medicine, Iran University of Medical Sciences, Tehran, Iran;4. Research Center for Immunodeficiencies, Children''s Medical Center, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;6. Cancer Immunology Project (CIP), Universal Scientific Education and Research Network (USERN), Stockholm, Sweden |
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| Abstract: | Pseudomonas aeruginosa is one of the most important infectious pathogens in medicine. This bacterium causes various infections, especially in patients with severe burns and people with defective immune systems. The purpose of this study was to develop a nanovaccine based on PLGA nanoparticles and lipopolysaccharide and oligopolysaccharide antigens for appropriate stimulation of the humoral and cellular immune systems against P. aeruginosa. LPS-PLGA and OPS-PLGA conjugates were synthesized using the carbodiimide reaction. The prepared conjugates of as well as the pure antigens of LPS and OPS were injected to BALB/c mice in three periods at 2 week intervals. The ELISA test showed that the IgM, IgA, IgG, IgG1, IgG2b, IgG2a and IgG3 antibodies produced against LPS-PLGA or OPS-PLGA conjugates were tens of times higher than the pure antigens. Also, the opsonophagocytosis test showed that the performance and the effect of produced anti-LPS-PLGA antibodies were higher than other groups. In addition, the mice treated with LPS-PLGA conjugate were more resistant to P. aeruginosa infection than other groups. These findings indicated that LPS and OPS antigens in conjugation with PLGA nanoparticles have the ability to create and effective immunity against P. aeruginosa and LPS-PLGA is more effective than OPS-PLGA. |
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| Keywords: | Lipopolysaccharide Oligopolysaccharide Immunoglobulin Opsonophagocytosis Challenge |
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