丙酸睾酮对大鼠胸腺细胞凋亡及胸腺中Bcl-2、Bax表达的影响

目的探讨丙酸睾酮在大鼠胸腺退化过程中的作用及其对大鼠胸腺中Bcl-2和Bax表达的影响。方法雄性大鼠去势后给予丙酸睾酮皮下注射,分别于注射后第1、4、7天取材,用半薄切片甲苯胺蓝染色检测胸腺细胞凋亡情况,免疫组织化学法检测胸腺组织中Bcl-2和Bax的表达情况,采用t检验进行统计学处理。结果丙酸睾酮处理组大鼠胸腺组织中可发现较多的凋亡细胞和凋亡小体;去势组大鼠胸腺组织中Bcl-2表达量明显高于假手术组（P〈0.001）,丙酸睾酮处理后Bcl-2表达量明显减少（P〈0.001）;而Bax的表达正好相反,去势组大鼠胸腺组织中Bax表达量明显低于假手术组（P〈0.001）,给予丙酸睾酮后Bax表达量明显增加（P〈0.001）;结论丙酸睾酮可诱导大鼠胸腺细胞凋亡,并抑制大鼠胸腺组织中Bcl-2的表达,促进Bax的表达。

Effect of Testosterone Propionate on Thymocytic Apoptosis and Bcl-2,Bax Expression in Rats

Objective To investigate the effects of testosterone propionate on thymocytic apoptosis and Bcl-2、bax expression in thymus tissuse of rats.Methods Male rats performed with bilateral orchidectomy were injected with testosterone propionate,thymus tissuses were obtained after 1、4 and 7 days operation.Thymocytic Apoptosis was detected by semimicrotomy and the expression of Bcl-2 and Bax in thymus was performed by immunohistochemistry.Results Apoptotic cells and apoptotic bodies were found more in thymus of the group treated with testosterone propionate than those of control group;Expression of Bcl-2 in orchidectomy group was higher than that in control group（P〈0.001）;when rats were injected with testosterone propionate,the expression of Bcl-2 was decreased obviously（P〈0.001）.Reversely,the Expression of Bax were lower in orchidectomy group than in contol group,while increased obviously when being administered with testosterone propionate.Conclusion Testosterone propionate probably induces the apoptosis of the thymocytes in male rats,restrains the Bcl-2 and promotes the Bax expression in thymus tissue.