Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity |
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| Authors: | Kang-Hsuan Chiu Keng-Liang Ou Sheng-Yang Lee Che-Tong Lin Wei-Jen Chang Chang-Chih Chen Haw-Ming Huang |
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| Institution: | (1) Department of Otolaryngology, Taipei Municipal Wan-Fang Hospital, Taipei, Taiwan;(2) Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan;(3) School of Dentistry, Taipei Medical University, Taipei, Taiwan;(4) Department of Emergency Medicine, Mackay Memorial Hospital, Taipei, Taiwan |
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| Abstract: | The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field
(SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the
changes in alkaline phosphatase activity and electron microscopy images. Membrane fluidity was used to evaluate alterations
in the biophysical properties of the cellular membranes after the SMF simulation. Our results show that SMF exposure increases
alkaline phosphatase activity and extracellular matrix release in MG63 cells. On the other hand, MG63 cells exposed to a 0.4-T
SMF exhibited a significant increase in fluorescence anisotropy at 6 h, with a significant reduction in the proliferation
effects of growth factors noted at 24 h. Based on these findings, the authors suggest that one of the possible mechanisms
that SMF affects osteoblastic maturation is by increasing the membrane rigidity and reducing the proliferation-promoting effects
of growth factors at the membrane domain. |
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| Keywords: | Mechanobiology Growth factor Fluorescence anisotropy SMF |
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