| 毒热平注射液对流感病毒感染的巨噬细胞分泌NO的影响 |
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| 引用本文： | 张艳丽,王宁萍,顾立刚,李澎涛.毒热平注射液对流感病毒感染的巨噬细胞分泌NO的影响[J].中国免疫学杂志,2010,26(5). |
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| 作者姓名： | 张艳丽 王宁萍 顾立刚 李澎涛 |
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| 作者单位： | 1. 宁夏医科大学病原生物学与免疫学系,银川,750004
2. 北京中医药大学中医药抗病毒教育部重点实验室,北京,100029 |
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| 基金项目： | 宁夏医科大学特殊人才启动项目 |
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| 摘 要： | 目的:研究毒热平注射液对流感病毒感染的小鼠腹腔巨噬细胞株Ana-1细胞分泌NO的影响。方法:用100TCID50流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠腹腔巨噬细胞株Ana-1后换用6个不同浓度的含药维持液继续培养,分别于6、12、24和36小时收集细胞上清液,用Griess试剂法检测其释放NO的水平。同时提取1μg/ml药物组作用24小时后的细胞mRNA和核蛋白,利用RealTimePCR法和Westernblot技术,检测毒热平注射液对病毒感染前后巨噬细胞NF-κBp65mRNA及其蛋白表达水平的影响。结果:在药物作用的不同时间,60、30和10μg/ml各剂量组均能显著下调病毒感染巨噬细胞NO的分泌水平,且具有剂量依赖性;该药可明显下调病毒感染巨噬细胞NF-κBp65mRNA及其蛋白表达水平。结论:毒热平可通过下调细胞核因子-κB活性,抑制病毒感染巨噬细胞NO的分泌,发挥抗病毒感染作用。
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| 关 键 词： | 毒热平 流感病毒 巨噬细胞 NF-κB |
Effect of Dureping Injection on NO secreted by macrophage infected by influenza virus |
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| ZHANG Yan-Li,WANG Ning-Ping,GU Li-Gang,LI Peng-Tao.Effect of Dureping Injection on NO secreted by macrophage infected by influenza virus[J].Chinese Journal of Immunology,2010,26(5). |
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| Authors: | ZHANG Yan-Li WANG Ning-Ping GU Li-Gang LI Peng-Tao |
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| Institution: | ZHANG Yan-Li,WANG Ning-Ping,GU Li-Gang,LI Peng-Tao.Department of Etiology , Immunology of Ningxia Medical University,Yinchuan 750004,China |
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| Abstract: | Objective:To survey the effect of the Dureping Injection on the kinetic change of nitrous oxide(NO) in macrophage infected by the influenza virus FM1 strain.Methods:The murinal celiac macrophage(Ana-1) was infected by the virus,add the different concentration of the drug in the supernatant of the macrophage for 6 h,12 h,24 h and 36 h.The level of the NO in the supernatant was measured by the method of traditional Griess.Ana-1 cell line was infected by influenza virus FM1 strain,then treated with Dureping In... |
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| Keywords: | NO |
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