长链非编码RNA WIF1-1在食管鳞状细胞癌组织中的表达及其临床意义

目的:探究食管鳞状细胞癌组织及癌旁组织中长链非编码RNA(long noncoding RNA,lncRNA)WIF1-1的表达水平,并评估其对患者预后的价值.方法:使用实时荧光定量PCR检测50例食管鳞状细胞癌组织样本及其对应同源的癌旁组织样本中lncRNA WIF1-1的表达水平,分析临床病理特征及其与患者预后的相关性.结果:食管鳞状细胞癌组织中lncRNA WIF1-1的表达水平明显低于癌旁组织,差异有统计学意义(P<0.001).进一步分析发现:lncRNA WIF1-1的表达水平与食管鳞状细胞癌的淋巴结转移、TNM分期和临床分期之间呈负相关.对预后资料分析发现:lncRNA WIF1-1表达水平较低的食管鳞癌患者的总生存时间(overall survival,OS)以及无进展生存期(progression-free survival,PFS)均较短.单因素及多因素分析结果显示:lncRNA WIF1-1可作为食管鳞状细胞癌患者OS和PFS的独立危险因素.结论:lncRNA WIF1-1可能在食管鳞状细胞癌中发挥抑癌基因的作用,其表达量与患者预后具有相关性,有可能成为食管鳞状细胞癌的治疗靶点.

Expression and clinical significance of long noncoding RNA WIF1-1 in esophageal squamous cell carcinoma

Objective: To investigate the expression level of long noncoding RNA (lncRNA) WIF1-1 and its related prognostic value in esophageal squamous cell carcinoma (ESCC). Methods: The expressions of lncRNA WIF1-1 in 50 ESCC tissues and the corresponding normal tissues were detected via RT-qPCR method. The association between long non-coding RNA WIF1-1 expression and clinical characteristics was evaluated via Chi-square test. Results: The expression of WIF1-1 in ESCC tissues decreased significantly compared with that in the corresponding normal tissues, the difference was statistically significant (P<0.5). Furthermore, WIF1-1 was negatively related to the lymph nodes metastasis, TNM and clinical stage. Overall survival (OS) and progressionfree survival (PFS) were significantly shorter in ESCC patients with lower expression level of lncRNA WIF1-1. Moreover, the down-regulated WIF1-1 expression was significantly correlated with the shorter survival of ESCC patients. The results of univariate and multivariate analysis revealed the WIF1-1 expression acted as an independent prognostic factor both in OS and PFS. Conclusion: The lncRNA WIF1-1 can be an important and potential suppressor of ESCC, and the expression of WIF1-1 may serve as another promising therapeutic target and prognostic biomarker in future clinic.