Cystatin B在肝癌组织中的表达及其与患者预后的关联 |
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引用本文: | 程卓,黄磊,丁劲.Cystatin B在肝癌组织中的表达及其与患者预后的关联[J].国际病理科学与临床杂志,2016(5):545-549. |
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作者姓名: | 程卓 黄磊 丁劲 |
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作者单位: | 第二军医大学附属东方肝胆外科医院,国际合作生物信号转导研究中心,上海 200433 |
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摘 要: | 目的:研究Cystatin B(CSTB)在肝癌组织中的表达及其与肝癌患者预后的相关性。方法:利用Real-time PCR和Western blot分别检测肝癌病人癌与癌旁组织中CSTB mRNA水平和蛋白的表达,进一步通过免疫组织化学法检测组织芯片中CSTB表达并分析其与肝癌临床特征的关系。采用Kaplan-Meier生存分析法分析CSTB与肝癌预后的关系。结果:在肝癌组织中CSTB mRNA水平和蛋白水平的表达明显高于癌旁(P<0.05);生存分析发现CSTB高表达肝癌患者比低表达的患者更易复发且总体生存期短(P值均小于0.05);CSTB的表达与年龄、性别、BCLC分级以及肿瘤大小差异均无统计学意义(P值均大于0.05),CSTB高表达的患者更容易形成门脉癌栓(P<0.05)。结论:CSTB在肝细胞癌组织中高表达,其表达水平与肝癌病人预后有关,有望成为肝癌预后指标。
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关 键 词: | 肝细胞癌 CSTB 预后 |
Expression and prognostic value of Cystatin B in hepatocellular carcinoma |
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Abstract: | Objective: To investigate the expression of Cystatin B (CSTB) in hepatocellular carcinoma (HCC) and its prognostic value.Methods: hTe expression of CSTB in HCC patients’ tumor tissues and paracancerous tissues was measured by real-time-PCR, Western blot and immunohistochemical staining, and the associations between CSTB and clinicopathologic features of HCC were analyzed. hTe prognostic value of CSTB was estimated using Kaplan-Meier approach.Results: hTe expression of CSTB in HCC’s tumor tissues was signiifcantly higher than that in adjacent normal tissues (P<0.05). HCC patients with high CSTB expression displayed higher risk of recurrence and shorter survival time (P<0.05). CSTB did not correlate with age, gender, BCLC grades and tumor sizes (P>0.05 for all). However, higher CSTB was associated with the incidence of portal vein tumor thrombus (PVTT) (P<0.05).Conclusion: CSTB was highly expressed in HCC and correlated with poor prognosis of patient, and it is a potential prognostic marker for HCC. |
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Keywords: | hepatocellular carcinoma Cystatin B prognosis |
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