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长链非编码RNA uc.299在心脏发育中的作用
引用本文:朱晓静,程子杰,冯梦文,尹安雯,沈丹,王兴,李沄.长链非编码RNA uc.299在心脏发育中的作用[J].国际病理科学与临床杂志,2017,37(6).
作者姓名:朱晓静  程子杰  冯梦文  尹安雯  沈丹  王兴  李沄
作者单位:1. 南京中医药大学附属中西医结合医院病理科,南京,210028;2. 南京医科大学第一附属医院心血管内科,南京,210029;3. 南京市妇幼保健院医学研究中心,南京,210004;4. 南京市妇幼保健院药剂科,南京,210004
基金项目:国家自然科学基金(81601333).This work was supported by the National Natural Science Foundation of China
摘    要:目的:研究长链非编码RNA(long non-coding RNA,lncRNA)uc.299在P19细胞分化及斑马鱼心脏发育中的作用,探讨其与心脏发育之间的关系.方法:体外实验中,通过shRNA沉默uc.299后,检测对P19分化的影响;在体实验中,对斑马鱼受精卵显微注射uc.299反义寡核苷酸,观察对斑马鱼心脏发育的影响.结果:与对照组相比,沉默uc.299对P19细胞分化有明显的抑制作用,心肌细胞跳动频率降低,各心肌标志物表达水平有明显下调.斑马鱼实验表明,沉默uc.299可造成斑马鱼心脏明显水肿.结论:沉默uc.299可影响P19分化和斑马鱼心脏发育,表明uc.299对维持心脏正常发育具有一定作用.

关 键 词:长链非编码RNA  室间隔缺损性先天性心脏病  P19  斑马鱼  作用

Effect of long non-coding RNA uc.299 on heart development
ZHU Xiaojing,CHENG Zijie,FENG Mengwen,YIN Anwen,SHEN Dan,WANG Xing,LI Yun.Effect of long non-coding RNA uc.299 on heart development[J].Journal of International Pathology and Clinical Medicine,2017,37(6).
Authors:ZHU Xiaojing  CHENG Zijie  FENG Mengwen  YIN Anwen  SHEN Dan  WANG Xing  LI Yun
Abstract:Objective:To investigate the function oflncRNA uc.299 in the heart development of zebra fish and differentiation of P19 cells.Methods:In in vitro experiment,the effect of uc.299 knockdown on P19 cells differenctiation were detected;and in vivo,after macroinjecting the zygotes zebra fish with uc.299 antisense oligonucleotide,the cardic development of the zebra fish was observed.Results:The differentiation and related cardiac biomarkers of P 19 are reduced as well as the heart development of zebrafish post Knockdown of uc.299.Conclusion:The differentiation and development are influenced post knockdown of uc.299.It indicates that uc.299 may play a role in maintaining heart development.
Keywords:long non-coding RNA  ventriculap septal defect  P19  zebra fish  function
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