肺癌胸水间期细胞遗传学异常变化的研究

目的 探讨肺癌胸水肿瘤细胞的间期细胞遗传学改变，并将其结果与传统细胞学结果进行比较。方法 用７号、１１号、１７号、Ｘ染色体着丝粒特异的ＤＮＡ探针，以２６例肺癌胸水标本进行双色荧光原位杂交（ｆｌｕｏｒｅｓｃｅｎｃｅ ｉｎ ｓｉｔｕ ｈｙｂｒｉｄｉｚａｔｉｏｎ，ＦＩＳＨ）结果 在１９例肺癌阳性胸水标本中，７号、Ｘ、１７号、１１号染色体出现超二倍体的病例分别为１６例（８４．２％）、１４例（７３．７％）、

Study on interphase cytogenetic abnormalities in malignant cells in pleural fluids from lung cancer cases

Objective To study the interphase cytogenetic abnormalities in malignant cells in pleural fluids from lung cancer cases by fluorescence in situ hybridization(FISH) and compare the result of FISH with that of conventional cytology. Methods Twenty six pleural fluids from the lung cancer cases were detected by dual fluorescence in situ hybridization centromere DNA probes of chromosomes 7, 11, 17, and X. Results In 19 positive pleural fluids the rates of hyperdiploid in chromosomes 7, X, 17 and 11 were 16(84.2%), 14(73.7%), 12(63.2%) and 8(42.1%), respectively. In 2 suspicious malignant pleural fluids, both chromosomes 7 and X showed hyperdiploid. Two cases of hyperplasia from 5 negative specimens also showed gain of chromosome X. Another 3 negative pleural fluids had a normal number of chromosome. Conclusion One of the important abnormalities occurred in tumor cells derived from pleural fluid of lung cancer is hyperdiploid, which can be detected by FISH. Therefore, hyperdiploid in pleural fluid cells can be an implication for the malignancy that has not been detected or confirmed by cytological examination.