山东汉族群体15个常染色体短串联重复序列的遗传多态性分析

目的 获取15个短串联重复序列(short tandem repeat,STR)基因座在山东汉族群体的群体遗传学数据.方法 应用AmpFISTR IdentifilerTM Kit试剂盒对15个SIR进行分型.结果 15个基因座的观察杂合度为0.605～0.882,期望杂合度为0.625～0.862,多态信息量为0.57～0.85,个体识别力为0.795～0.958,非父排除率为0.297～0.758.TPOX的等位基因频率,山东汉族群体与河南、吉林、江苏汉族群体间的非差异P值大于0.05;D13S317的等位基因频率,山东汉族群体与鄂温克族群体的P值大于0.05.结论 15个STR基因座在该群体多态性,除TPOX和TH01,其余13个STR基因座在山东汉族群体的非父排除率和累计个人识别机率较高.

Analysis of the genetic polymorphisms of 15 autosomal short tandem repeat in a Han population of Shandong

Objective To obtain population genetic data at 15 short tandem repeat(SIRs) in Shandong Han population.Methods Fifteen STRs were genotyped in 200 unrelated individuals from Shandong Han population using IdentifilerTM Kit.Results In this Shandong Han population,the observed hetertrzygusities of the 15 loci ranged from 0.605 to 0.882,and the expected heterozygosities were 0.625-0.862,the polymorphic information contents ranged from 0.57 to 0.85,the discrimination powers ranged from 0.795 to 0.958,and the exclusion probabilities ranged from 0.297 to 0.758.The non-differentiation exact P values at TPOX loci were more than 0.05 between Shandong and Han population of Henan,Jilin,and Jiangsu.The non-differentiation exact P values between Shandong Han population and others populations were more than 0.05 at others loci.The P value at D13S317 was more than 0.05 between Shandong Han population and Ewenki population.Condusien These loci are polymorphic in Shandong Han population,and the discrimination powers and exclusion probabihties were high in the 15 STRs except TPOX and TH01.