| 脉冲电刺激对血管内皮细胞与其祖细胞黏附的影响 |
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| 作者姓名： | Li W Zheng L Wang Q Guo S |
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| 作者单位： | 南方医科大学南方医院检验医学中心; |
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| 基金项目： | 广东省自然科学基金资助项目(07300312); 国防科工委专项课题资助项目(B3320062101); 南方医院院长基金资助项目(2009Z004) |
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| 摘 要： | 为探索脉冲电刺激下血管内皮细胞与内皮祖细胞(EPC)之间黏附强度的改变,诱导培养外周血EPC,荧光标记后与单层血管内皮细胞共培养,固定电压和频率为5 V和5 Hz,选择1、3、6、9 ms的脉宽对其进行干预,持续刺激24 h后检测贴壁EPC的荧光强度,以荧光比率衡量。结果显示,与对照组相比,3 ms刺激组荧光比率即显著增高,随着脉宽延长,6 ms组达到最大值,但9 ms刺激组却显著下降,提示适宜脉冲电刺激有利于血管内皮细胞与EPC之间的黏附,为电刺激促进血管新生的研究提供新的理论依据。
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| 关 键 词： | 脉冲电刺激 血管新生 血管内皮细胞 内皮祖细胞 黏附 |
Effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell |
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| Li W,Zheng L,Wang Q,Guo S.Effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell[J].Journal of Biomedical Engineering,2011,28(4):689-93, 697. |
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| Authors: | Li Weiqi Zheng Lei Wang Qian Guo Shuyan |
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| Institution: | Li Weiqi1 Zheng Lei1 Wang Qian1 Guo Shuyan21(Department of Clinical Laboratory Center,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)2(School of Biomedical Engineering,China) |
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| Abstract: | The present paper is aimed to investigate the effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell(EPC).EPC was induced from periphery blood,labeled with fluorescence dye and then co-cultured with vascular endothelial cell.With a fixed electric voltage and frequency of 5V and 5Hz,respectively,the co-culture system was continually stimulated for 24h under different pulse width,1,3,6 and 9ms.After pulse stimulation,fluorescence intensity of ad... |
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| Keywords: | Pulse electrical stimulation Neovascularization Vascular endothelial cell Endothelial progenitor cell(EPC) Adhesion |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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