碱性成纤维细胞生长因子对成骨细胞生长与c-fos表达的影响

以重组牛碱性成纤维细胞生长因子 (rb- b FGF)刺激体外培养的大鼠成骨细胞 ,发现 :经过 1～ 2 d的刺激 ,成骨细胞产生很长的突起 ,细胞数量和活力较对照均有显著升高 ;经 rb- b FGF 1h的刺激后 ,c- fos基因的表达即明显高于对照 ,刺激 1～ 2 d后 c- fos基因的表达也明显高于对照。这表明 b FGF可促进大鼠成骨细胞增殖 ,提高c- fos基因的表达量。 c- fos表达量的提高 ,可能是各种刺激在促进细胞增殖的信号转化过程中的重要一步

Effects of Recombinant Bovine Basic Fibroblast Growth Factor on Osteoblast's Growth and c-fos Expression

Rat's osteoblasts cultured in vitro were stimulated by recombinant bovine basic fibroblast growth factor(rb-bFGF).After 1-2 days,the osteoblast grew long protuberance, the numbers of osteoblasts were greater than the control groups', the vitality of osteoblasts was better than that of control groups.After 1 hour,the expression of c-fos in osteoblast increased when compared with that in the control group. After the osteoblasts having been stimulated for 1-2 days, the expression of c-fos increased more conspicuously.These results show that bFGF can boost the osteoblast to grow and proliferate and can enhance the expression of c-fos gene.The increase of c-fos gene's expression may be an important step in the singal transformation process of all kinds of stimulation boosting osteoblast to proliferate.