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脉冲电刺激对内皮细胞形态和功能的影响
引用本文:乐安,杨刚,吴江,赖怡,黄华,陈槐卿.脉冲电刺激对内皮细胞形态和功能的影响[J].生物医学工程学杂志,2008,25(3):694-698.
作者姓名:乐安  杨刚  吴江  赖怡  黄华  陈槐卿
作者单位:1. 四川大学,华西医学中心,生物医学工程研究室,成都,610041
2. 四川大学,电气信息学院,医学信息学工程系,成都,610065
摘    要:探求脉冲电刺激对内皮细胞的形态、增殖以及蛋白表达的影响.选择在25 mV,频率分别为1、5、10、20、50和100 Hz的电刺激6 h作用于内皮细胞.MTT检测,分析细胞增殖.检测NO和ET-1值,分析内皮细胞蛋白表达.结果发现在10~100 Hz脉冲电刺激作用下,内皮细胞增殖明显.NO的表达在20~100 Hz电刺激作用下明显高于对照组.在1 Hz脉冲电刺激作用下ET-1的分泌量低于对照组,而20~100 Hz时则明显高于对照组.可以看出,脉冲电刺激对内皮细胞的增殖和NO、ET-1表达都产生了影响.尤其是50 Hz脉冲电刺激对于加强内皮细胞功能、维持血管生物学特性有着积极的作用.

关 键 词:脉冲电刺激  内皮细胞  增殖  脉冲电刺激  内皮细胞  形态和功能  影响  Endothelial  Cells  Functions  Morphology  Electrical  Stimulation  生物学特性  皮细胞功能  加强  细胞的增殖  分泌量  对照组  蛋白表达  电刺激作用  发现  结果  细胞增殖  分析

The Influence of the Pulsed Electrical Stimulation on the Morphology And the Functions of the Endothelial Cells
Yue An,Yang Gang,Wu Jiang,Lai Yi,Huang Hua,Chen Huaiqing.The Influence of the Pulsed Electrical Stimulation on the Morphology And the Functions of the Endothelial Cells[J].Journal of Biomedical Engineering,2008,25(3):694-698.
Authors:Yue An  Yang Gang  Wu Jiang  Lai Yi  Huang Hua  Chen Huaiqing
Institution:Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
Abstract:The aim of this study is to explore the effects of the pulsed electrical stimulation (PES) on the morphology, the proliferation and the values of NO and ET-1 of the endothelial cells (ECs). We chose the different frequency PES (1, 5, 10, 20, 50, 100 Hz) with 25 mV to stimulate the ECs for 6 hours. We observed the cell's morphous by the scanning electron microscope (SEM) and detected the values of MTT, NO and ET-1. The proliferation of the ECs was obviously rose up under the PES from 10 to 100 Hz. But the PES inhibited the proliferation with the frequency lower than 10 Hz. After stimulated with PES (20 - 100 Hz), the NO expression of ECs were increased obviously, and the peak value was appeared at 50 Hz. The peak value of ET-1 was appeared at 100 Hz. The PES has significant effects on the ECs' morphology, proliferation and expression of NO and ET-1. Particularly, the 50 Hz PES plays a positive role to enhance the ECs' function and to maintain the vascular biology.
Keywords:
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