Rac1参与调节IL-8诱导的内皮细胞迁移

研究Rac1是否参与调节IL-8诱导的内皮细胞迁移.采用Transwell小室迁移率分析法,考察不同Matrigel稀释比例以及IL-8不同作用时间对内皮细胞迁移的影响;同时用RT-PCR法检测Rac1 mRNA的表达.结果表明,Matrigel稀释比例为1:2时与比例为1:3、1:8时细胞迁移数量有显著性差异,而比例为1:4、1:5和1:6时与其他各组没有显著性差异;故选用Matrigel稀释比例1:4进行后期实验,随着IL-8作用时间的增加,内皮细胞迁移数量也逐渐增加;IL-8作用6h后,Rac1 mRNA的表达较其他各时间组稍强.以上结果提示,Rac1参与调节IL-8诱导的内皮细胞迁移,本实验为进一步研究Rac1在IL-8诱导内皮细胞迁移中的作用奠定基础.

Rac1 Mediates the Migration of Endothelial Cells Induced by IL-8

This study sought to elucidate whether Rac1 mediates the migration of endothelial cell induced by IL-8. The Transwell chamber motility assay was conducted to disclose the effect of different matrigel dilution and different time of IL-8 treatment on the migration of endothelial cells. The mRNA of Rac1 was detected by RT-PCR. The results demonstrated that when the concentration of Matrigel was 1:2, there is significant difference on the amounts of migration cells than that of the concentration of 1:3 or 1:8; When the dilution of Matrigel was 1:4, 1:5 or 1:6, there is no significant difference on the amounts of migration cells than that of other dilution groups. So we choose the Matrigel concentration as 1:4. With the increase of IL-8 stimulation time, the cells which migrated from upper reservoirs to lower reservoirs progressively increased. After six hours stimulation by IL-8, the expression of Rac1 mRNA in migrated cells was increased, compared with that of other groups. The results suggest that Rac1 may mediate the migration of endothelial cells induced by IL-8. It can also be the foundation for further investigation on the role of Rac1 in the migration of endothelial cells induced by IL-8.