PCR—DIG探针斑点杂交法快速检测SRY基因

以正常男性ＤＮＡ作为模板，用本文设计的ＳＲＹ基因的１对引物组合进行ＰＣＲ法制备ＤＩＧ探针，然后通过低熔点琼脂糖电泳回收将扩增产物纯化，得到ＳＲＹ基因探针。与其他制备探针的方法相比，此方法制得的探针产量和标记效率都很高，标记灵敏度达００１ｐｇ，片段长度均一，为２９０ｂｐ。在ＤＮＡ斑点杂交检测ＳＲＹ基因时显示了很好的特异性，显示这种方法可以同时对大量性反转病例进行临床检测。

Apply the PCRDIG probe to quickly detect the SRY gene by DNA dot blotting.

Complex genomic of human as template,using a pair combination of primers of SRY gene designed by us,we labeled the probe with DIG by PCR.After LMP agarose electrophoresis and purification,the probe has high yield,high labeling effect and high sensitivity,it's sensitivity limitation is 0.01pg,and the fragment's are the same which is 290 bp comparing to other labeling methods.The probe is specific when being used to hybrid to detect the existence of SRY gene,which suggest this method could be applied to detect many Sxr patients together for clinic use.