基因芯片分型与实时荧光PCR筛查成都地区妇女宫颈HPV-DNA感染结果比较

目的了解成都地区HPV亚型的感染情况;评价基因分型（genotyping）与实时荧光定量PCR（real-time PCR）两种人乳头瘤病毒（HPV）检测方法的差异性,为临床应用提供参考。方法对2012年1月-2013年6月间来院就诊的女性,其中3537名进行基因芯片检测,991名进行实时荧光PCR检测。所得数据用SPSS 17.0进行统计分析。结果基因芯片分型法检测出阳性1315例,阳性率37.18%;实时荧光PCR检测出阳性137例,阳性率13.82%,两种方法检出率差异明显。基因分型法显示,HPV6、11、16、58、43型位居前五;荧光定量PCR显示HPV6、11、16、52、58在前五位。结论成都地区低危感染以HPV 6、11型为主,高危以16型为主,次之52、58型;基因芯片分型与临床HPV-DNA筛查检出率高,优于荧光定量PCR法。

A comparison of genotyping and real-time PCR for the screening results of cervical HPV-DNA infection in Chengdu,China

Objective： The study is aim to investigate on HPV infection in Chengdu, China. And comparison of the screening results of cervical HPV-DNA infection between genotyping microarray and real-time PCR for clinical use. Methods： 3537 women, which were screened in January 2012 to June 2013, by genotyping while 991 women were by real-time PCR. The data were analyzed using SPSS 17.0. Results： 1315 patients with positive test and infection rate is 37.18% （95% CI, 35.59%~38.77%） by genotyping, and the infection rate by real-time PCR is 13.82% （95% CI, 11.67%~15.97%） with 137 positive patients. Conclusions： Low-risk of infection is mainly HPV6, 11, while the High-risk is HPV 16, followed HPV52 and 58 in Cbengdu, China. The screening results of cervical HPV-DNA infection between genotyping microarray and real-time PCR have a significant variability. Choice should be different when used in clinical.