人胎胃壁瘦素及瘦素受体的免疫组织化学

目的：对不同胎龄胎儿胃壁组织发育和胃壁内瘦素及瘦素受体进行定性和定位观察。方法：采用HE和免疫组织化学SABC染色方法。结果：胚胎13w，可见有胃小凹和胃腺。15w时，胃壁具有4层结构。随胎龄增长，胃壁和胃腺逐步发育完善。胚胎13w时，胎儿胃粘膜上皮和胃腺内壁细胞呈瘦素及瘦素受体免疫反应中等阳性，免疫反应产物分布于胞质，而胞核为阴性。随着胃腺的发育，呈免疫反应阳性的壁细胞数目增多。在胎儿整个发育过程中，各胎龄间胃腺瘦素及瘦素受体的免疫反应强度无明显变化。结论：人胚胎发育时期，在胃上皮和胃腺中有瘦素及瘦素受体的表达，推测它们对胎儿的生长发育起重要的调节作用。

Immunohistochemistry of leptin and leptin receptor in human fetus stomach

Objective:To observe the histogenesis, localization of leptin and leptin-receptor in stomach wall of 26 fetuses of different ages. Methods:Hematoxylin-eosin staining and immuno-histochemical SABC methods were used. Results:In 13-week fetus, gastric pit and gastric gland could be seen. The 15th week,the stomach wall formed tetra -layer structure,and the stomach wall and gastric gland developed with aging. In contrast to hematoxylin-eosin staining the leptin and leptin-receptor expressed in gastric epithelium and parietal cells of stomach on the 13th week fetus. The products of immunologic reaction distributed in cytoplasm,but nucleus was negative. The number of immunoreactive cells increased with the development of gastric gland.In the whole fetal period, the staining intensity was not obviously changed. Conclusion:The result indicates that there is close relation between leptin and development of human fetus.