脱细胞猪主动脉瓣叶的生物力学特性和免疫反应性

目的：探讨脱细胞猪主动脉瓣叶构建组织工程心脏瓣膜支架的可行性。方法：经胰酶-EDTA、表面活性剂、核酸酶处理，去除瓣叶的细胞成分，测定脱细胞瓣叶的生物力学特性，同时行大鼠皮下包埋实验，观察其免疫反应性。结果：瓣叶中的细胞成分能完全去除，获得无细胞的纤维网状支架。脱细胞瓣叶与新鲜瓣叶有基本相同的应力-应变曲线及应力-松弛曲线，而弹性模量、面积比、松弛强度、断裂强度和断裂伸长率两者无显著差异。脱细胞瓣叶的免疫反应性明显降低。结论：猪主动脉瓣叶经脱细胞处理后可以作为组织工程心脏瓣膜的支架材料。

Biomechanical characteristics and immunogenicity of acellularized porcine aortic valve leaflets

Objective: To investigate the feasibility of using acellularized porcine heart valve leaflet as a scaffold for tissue engineering. Methods: The porcine aortic valve leaflets were acellularized by trypsin-EDTA,Triton X-100,RNase and Dnase, and then its biomechanical characteristics was tested and the immunogenicity of acellularized valve leaflet implanted subcutaneous in rats was observed. Results:The valve leaflets were completely removed of the cell components and the construction of matrix was maintained. The biomechanical characteristics of the acellular valve leaflets and the fresh ones were almost similar. The immunogenicity of the acellularized valve leaflets was alleviated significantly compared with that of fresh ones. Conclusion:Acellularized porcine aortic valve leaflets can be applied to develop tissue engineering heart valve as a scaffold.