睫状神经营养因子对大鼠去神经骨骼肌的营养作用

目的：通过测定SD大鼠坐骨神经离断后比目鱼肌（SOL）和趾长伸肌（EDL）肌纤维横截面积，观察持续给睫状神经营养因子（CNTF）对神经骨骼肌萎缩的影响。结果：SD大鼠坐骨神经离断后，持续给0．2mg/kg.d的CNTF20天后，损伤侧SOL和EDL肌纤维横截面积分别比实验对照组高27％（P＜0．01）和14％，SOL肌纤维横截面积比EDL增加的幅度大，而给予0．05mg/kg.d的CNTF20天后，动物肌纤维横截面积与实验对照组无明显差异。结论：CNTF可显著改善坐骨神经离断后SD大鼠骨骼肌的萎缩，并且CNTF效应的强弱与用药剂量和肌肉类型有关，0．2mg/kg.dCNTF作用明显强于0．05mg/kg.dCNTF，慢肌（SOL）比快肌（EDL）对CNTF更敏感。

EFFECT OF CILIARY NEUROTROPHIC FACTOR ON DENERVETED SKELETAL MUSCLE ATROPHY

The effect of ciliary neurotrophic factor (CNTF) on the denervetde skeletal muscle atrophy was studied by detecting fiber cross-sectional areas soleus muscle (SOL) and extensor digitorum longus (EDL) in nerve transected SD rats.SD rats with sciatic nerve transected were administrated CNTF (0.2mg/kg)continuously for 20 days.It were found that in the fiber cross-sectional areas in injury side,SOL and EDL raised by 27% ( P <0.01) and 14%,with a bigger increase in SOL fiber cross-sectional areas than EDL compared with that of model group.But no obvious changes was observed in animal administrated with CNTF of 0.05 mg/kg.CNTF attenuated skeletal muscle atrophy significantly in sciatic nerve transected SD rats,so it could be said that CNTF had atrophic effect on denervated skeletal muscle.The effect may be in a dosage dependent manner.And it was related with the type of muscle,because the myotrophic effect of 0.2 mg/kg CNTF was more effective than that of 0.05 mg/kg CNTF,and slow twitch muscle (SOL) reacts to CNTF stronger than quick twitch muscle did (EDL).