微囊化兔雪旺细胞移植对大鼠损伤脊髓胶质纤维酸性蛋白表达的影响

目的:观察大鼠脊髓损伤后及进行微囊化兔雪旺细胞移植后胶质纤维酸性蛋白(GFAP)表达的变化.方法: 130只成年SD大鼠随机分为微囊组、细胞悬液组、损伤对照组和正常对照组4组,术后3、 7、 14及28d,冰冻切片行免疫组织化学显色观察GFAP表达的变化.结果:大鼠脊髓损伤后3～14d, GFAP阳性细胞数及平均光密度均增加;至第28天时则减少,但仍高于正常组.其中阳性细胞数和平均光密度在第7天开始,微囊组与细胞悬液组、损伤组比较均有明显降低.结论: 微囊化异种雪旺细胞移植能抑制损伤脊髓GFAP的表达,减轻由反应性胶质化所形成的胶质瘢痕.

Effect of xenotransplantation of microencapsulated Schwann cells on glial fibrillary acidic protein expression in the injured spinal cord of adult rat

Objective: To study the changes of glial fibrillary acidic protein (GFAP) expression after spinal cord injury (SCI) and the changes after transplantation of microencapsulated Schwann cells. Methods: 120 adult Sprague-Dawley rats were randomly and equally divided into microcapsule group, cell suspension group, injury group and control group. Additional 10 rats were used as normal controls. The expression of GFAP in sections of rat spinal cord was detected using immnohistochemical technique at 3, 7, 14 and 28 days after injury. Results: After SCI, the number of GFAP positive cells and mean optical density of GFAP were raised from day 3 to day 14, and decreased on day 28, which was still higher than normal. Compared with cell suspension and injury groups, xenotransplantation of microencapsulated Schwann cells produced a significant decrease in the number of GFAP positive cells and mean optical density of GFAP. Conclusion: Xenotransplantation of microencapsulated Schwann cells can downregulate the expression of GFAP in astrocytes of the injured spinal cord, therefore, reduce glial scar caused by reactive astrogliosis.