单宁酸—金属盐联用媒染脑血管的研究

用２％多聚甲醛，２％戊二醛配伍不同浓度的单宁酸媒染固定液灌流大鼠，取服切片，放入２％氯化铁溶液中呈色，成功地显示了脑内各级血管及毛细血管网的形貌。高倍镜下可见各级血管呈空心管道状，基僻赤多条细纤维丝状结构编织或呈同心圆状排列而成，具有较强的立体感。

A STUDY OF STAINING BLOOD VESSELS WITH TANNIC ACID

The rats were perfused with compound staining-fixative solution of 2% paraformaldehyde, 2% glutaraldehyde and various concentration of tannic acid(0.5-5% TA). The specimens from the brain were cut into slices and immersed in 2% ferric chlorid solution to reveal colors by che-mical reactions. The capillary network and arterioles were well manifested as vacuous tubes under light microscope- The wall of the vessels was formed by many woven or concentrically arranged slender fibers, creating distinct three-dimensional sensation. The crossing, overlapping and anastomosis of capillaries and arterioles were be seen clearly. The findings suggest that the effect of staining was best with the 2-3% concentration of tannic acid. The study provided an ideal, simple and direct method for further investigating microvasuclar and pathololgical changes.