免不方治疗免疫性不育雌鼠的组织学和免疫组化

目的：探讨中药复方免不方治疗抗精子抗体引起的免疫性不育雌小鼠卵巢子宫组织学、免疫组化变化。方法：用精子抗原免疫昆明种雌鼠，建立免疫性不育动物模型；同时分别饲喂中药复方免不方１号、２号，醋酸强的松，生理盐水；主要从组织学、免疫组化观察免疫性不育症的变化。结果：免疫性不育症鼠血清，宫颈粘液抗精子抗体高，卵巢和子宫内膜免疫复合物沉积多，卵巢闭锁卵泡多；中芭能降低卵巢子宫内膜免疫复合物沉积，降低血清和宫颈粘液抗精子抗体。结论：免不方通过调节全身免疫系统，吸收，清除循环和局部抗精子抗体，免疫复合物，降低和修复组织损伤，从而提高小鼠的妊娠。

HISTOLOGY AND IMMUNOHISTOCHEMISTRY STUDY ON MIANBUFAN TREATING IMMUNOLOGICAL INFERTILITY OF FEMALE MICE

Objective: To explore the histology and immunohistochemistry change of ovary and endometrium after Chinese Medicine Mianbufan healing immunological infertility in female mice causing by antisperm antibody (AsAb). Methods: By injection of mice sperm, model mice of immunological infertility were established. The mice were treated with Mianbu I, Mianbu II or Prednisone Acetatis when model werer established. The characteristic of histology and immunohistochemistry of mice were analyzed. Results: The level of AsAb was high in serum and cervical mucus of model mice. Immune compounds were mostly deposited in ovary and endometrium. The atretic follicle of ovary was much in amount. However, the Chinese medicine could significantly reduce the level of AsAb, even eliminate it and clear the deposition of immune compounds of ovary and endometrium. Conclusion: Chinese medicine Mianbu Fan Can regulate, absorb, clear circulating and parting AsAb and immunological compound, reduced and repair tissue impairment and increase the pregnancy of female mice.