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Scanning Microscopy of Colonic Mucin during Carcinogenesis: Is It Clinically Applicable? |
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| Authors: | James L Weese Kennedy W Gilchrist Ralph M Albrecht |
| Institution: | a Department of Surgery/Human Oncology, University of Wisconsin Center for Health Sciences, Madison, Wisconsin b Department of Surgical Oncology, Fox Chase Cancer Center, Temple University School of Medicine, Philadelphia, PA c Section of Surgical Oncology, Temple University School of Medicine, Philadelphia, PA d Department of Pathology/Human Oncology, K4/536 Clinical Science Center, University of Wisconsin Center for Health Sciences, Madison, Wisconsin e University of Wisconsin Center for Health Sciences, Veterinary Science/Pediatrics, Madison, Wisconsin |
| Abstract: | The integrity of the colonic mucin layer has been reported to be altered during carcinogenesis in both humans and rodents. Prior to attempting scanning microscopic techniques on colonic mucosa of patients at high risk to develop colorectal cancer, these procedures were performed on colonic mucosa from rats with chemically induced colon cancers. Substantial technical difficulties in preparation and serious subjectivity in interpretation of the scanning micrographs were encountered. The major technical problem was the unpredictable retention of the mucin layer upon both normal and cancerous mucosae. Visual interpretation of the integrity or disruption of the mucin layer with the scanning electron microscope revealed variable fenestration and fraying of the mucin in both normal and cancerous colons. Our findings suggest that scanning electron microscopy of colonic mucin may not be a reliable screening procedure for (pre)cancerous changes in human colonic mucosae. |
| Keywords: | scanning microscopy colonic mucin cancerous alterations clinical applicability |
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