| 几种不同基因免疫途径的比较研究 |
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| 引用本文： | 段明星,庄峰锋.几种不同基因免疫途径的比较研究[J].现代免疫学,1999,19(5):275-276,279. |
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| 作者姓名： | 段明星 庄峰锋 |
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| 作者单位： | 清华大学生物科学与技术系!北京100084 |
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| 摘 要： | 以乙肝表面抗原基因疫苗为模型， 采用肌肉注射、腹腔注射、表皮划痕三种免疫途径和不同剂量的裸露质粒ＤＮＡ免疫小鼠， 以ＥＬＩＳＡ方法检测小鼠血清中抗ＨＢｓＡｇ 抗体变化。实验结果表明表皮划痕方式免疫小鼠免疫反应阳性率高，且在相同剂量下其抗体滴度水平最高； 肌肉注射方式免疫小鼠， 抗体反应可维持较长时间； 腹腔注射方式免疫小鼠， 抗体反应产生快， 但维持时间较短。
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| 关 键 词： | 核酸疫苗 乙肝表面抗原 免疫途径 |
A Comparative Study on the Inoculation Routes of Gene Immunization |
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| Duan Mingxing,Zhuang Fengfeng,Zhou Zhihong,Zhang Fang,Chang Zengyi,Zheng Changxue..A Comparative Study on the Inoculation Routes of Gene Immunization[J].Current Immunology,1999,19(5):275-276,279. |
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| Authors: | Duan Mingxing Zhuang Fengfeng Zhou Zhihong Zhang Fang Chang Zengyi Zheng Changxue |
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| Abstract: | DNA based immunization against hepatitis B surface antigen was used as a model system for the studies of the immune responses Mice were immunized with genes encoding the hepatitis B surface antigens by intramuscular,intraperitoneal,and epidermal scratch routes The changes of anti HBsAg antibodies in the sera of mice were tested by ELISA It was found that the plasmid DNA induced the highest level of antibody responses by epidermal scrach route over those of intramuscular and intraperitoneal inoculations in mice |
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| Keywords: | gene immunization hepatitis B surgace antigen inoculation route |
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