抗人IgG单克隆抗体的研究——Ⅰ.分泌抗人IgG单克隆抗体杂交瘤细胞株的建立

本文报道用人IgG免疫BALB/c 小鼠的脾细胞与骨髓瘤细胞系SP2/O融合,获得 3株分泌抗人 IgG单克隆抗体的杂交瘤细胞株,命名为 SIBPH1、SIBPH2和 SIBPH3。现重点报道SIBPH1株。用琼脂双扩散和免疫电泳等方法,证实该株杂交瘤细胞所分泌的单克隆抗体,对IgG具有良好的专一性,注入同系小鼠腹腔可诱生含较高效价抗IgG的腹水(双扩散1:256～512),单克隆抗体属小鼠IgG_1亚类,该杂交瘤细胞株经组织培养传代逾半年,冻存12个月,复苏良好,分泌抗IgG性能稳定。本文还对杂交瘤细胞建株的若干问题进行了讨论。

STUDIES ON THE MONOCLONAL ANTIBODIES AGAINST HUMAN IgG I. THE ESTABLISHMENT OF THE HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST HUMAN IgG

We succeeded in the establishment of three hybridoma cell lines secreting anti-human IgG monoclonal antibodies by means of the fusion between mouse myeloma cells SP2/O and spleen cells from BALB/c mice immunized with purified human IgG. One of the hybridoma cell lines was named SIBPII1. The McAb secreted by SIBPH1 showed high specific activity with immunodiffusion and hnmunoelectrophoresis techniques. SIBPH1 could produce ascitic fluids with high liter McAb after being inoculated into BALB/c mice I. P. (I. D. 1: 256-512). The McAb of SIBPH1 is of mouse IgG1 subclass.This cell line was cloned 4 times successively by limiting dilution method. Karyotype analysis of the subclones showed that the number was 95 ±3.SIBPH1 is stable to secrete anti-human IgG McAb in tissue culture over six months and having been stored liquid nitrogen over twelve months. Some problems of the established cell line were briefly discussed.