人眼睑板腺和Zeis腺性激素受体定性定位的免疫组织化学研究

目的探讨性激素受体在睑板腺和Zeis腺的定性和定位分布。方法采用免疫组织化学技术和DAB显色方法显示雌激素受体（ER）、孕激素受体（PR）、雄激素受体（AR）在睑板腺和Zeis腺上皮的表达。结果胎儿4个月睑板腺和Zeis腺上皮呈ER、PR、AR阳性；而儿童至成人组睑板腺和Zeis腺上皮呈ER、AR阳性。各种性激素受体定位同时出现在细胞膜、细胞质和细胞核。ER在睑板腺和Zeis腺上皮阳性表达率在年龄组间比较，均无显著性差异（P〉0．05）；PR在睑板腺和Zeis腺上皮阳性表达率在年龄组间比较，均有显著性差异（P〈0．05）：AR在睑板腺上皮阳性表达率在年龄组间比较无显著性差异（P〉0．05），而Zeis腺上皮阳性表达率则有显著性差异（P〈0．05）。ER、PR、AR阳性表达率在胎儿组性别间比较和ER、AR阳性表达率在儿童至成人组性别间比较均无显著性差异（P〉0．05）。结论胎儿4个月睑板腺和Zeis腺上皮ER、PR、AR即有表达。出生后睑板腺和Zeis腺上皮仅存在ER和AR，以ER为主。ER、PR、AR定位在细胞膜、细胞质和细胞核。

IMMUNOHISTOCHEMICAL STUDY ON QUALITATIVE IDENTIFICATION AND LOCALIZATION OF SEX HORMONE RECEPTORS IN MEIBOMIAN GLANDS AND ZEIS GLANDS

Objective To study both qualitative identification and localization of sex hormone receptors in meibomian glands and Zeis glands in healthy human. Methods By means of immunohistochemical technique and the method stained by DAB,a qualitative identification and localization of estrogen receptors(ER),progesterone receptors(PR) and androgen receptors(AR) were performed to reveal the expression in epithelial cell of meibomian glands and Zeis glands. Results Meibomian glands and Zeis glands in fetus of 4 months were positive in ER,PR,AR;Meibomian glands and Zeis glands from children to adult were positive in ER and AR.Each of receptor was located on cell membrane,and in plasma and nuclear.Analyses of fisher test showed no statistically significant different in ER expression between the age groups(P>0.05).It has statistically significant difference in PR expression between the age groups studied(P<0.05) and no statistically significant difference in AR expression of meibomian glands between the age groups(P>0.05),AR expression in Zeis glands between the age groups studied(P<0.05).ER,PR,AR expression in sex groups of the fetus and in ER,AR expression in sex groups from children to adult(P>0.05).Conclusion Meibomian glands and Zeis glands in fetus of 4 months expressed in ER,PR,AR;ER and AR are expressed from children to adult,mainly in ER.All of receptors are located on membrane,in plasma and nuclear as well.