利用Micro-PET显像技术分析阿尔茨海默病小鼠模型的脑糖代谢

目的 利用小动物PET（Micro-PET）影像技术18氟-脱氧葡萄糖（¹⁸F-FDG）摄取率分析阿尔茨海默病(AD)小鼠模型的脑糖代谢，探索评价治疗AD药物的影像分析技术。 方法 正常对照组、APP^swe/PSΔE9转基因模型组和安理申［2 mg/(kg•d)］治疗组小鼠共9只，自腹腔注射放射性示踪剂¹⁸F-FDG，采集脑部Micro-PET图像，通过软件IRW计算并比较各组小鼠大脑与额叶、颞叶感兴趣区（ROI）每克组织¹⁸F-FDG的摄取率。 结果 利用¹⁸F-FDG 的Micro-PET影像分析可以检测AD小鼠模型的脑糖代谢，并发现APP^swe/PSΔE9转基因小鼠的脑糖摄取明显降低，与人类AD患者表现的脑糖摄取降低一致，安理申治疗可以提升APP^swe/PSΔE9转基因小鼠的脑糖摄取。 结论 ¹⁸F-FDG Micro-PET影像分析可以用于小鼠AD模型脑葡萄糖代谢水平的检测，并可以作为阿尔茨海默病治疗药物提升脑糖代谢作用的分析技术。

Analysis of cerebral glucose metabolism in mouse of Alzheimer's disease model by Micro-PET

Objective To evaluate the cerebral glucose metabolism of Alzheimer’s disease(AD) model mouse by SUP>18/SUP>F-FDG and Micro PET，and develop an imaging technique to evaluate AD drugs therapeutic effect on animal models. Methods The 9 mice of control group, APPSUP>swe/SUP>/PSΔE9 double transgenic group and aricept treatment group［2 mg/(kgI>&#/I>8226;d)］ were injected SUP>18/SUP>F-FDG by peritoneal cavity and scanned by Micro-PET. The glucose uptake value of cerebrum，frontal cortex and temporal cortex region of interest(ROI) were analysed by IRW software. Results The images of SUP>18/SUP>F-FDG and Micro-PET could be used to detect the cerebral glucose metabolism of AD mouse model. We found that the cerebral glucose metabolism was decreased in the APPSUP>swe/SUP>/PSΔE9 double transgenic mice, which was consistent with phenotype of the human AD. Aricept treatment improved the cerebral glucose metabolism of AD disease model mice. Conclusion The imaging analysis using SUP>18/SUP>F-FDG an