褪黑素抑制胃癌细胞系SGC-7901的迁移和侵袭

目的 探讨褪黑素(MLT)对胃癌细胞迁移和侵袭的抑制作用及其分子机制。 方法 不同浓度(0.1、1、2 和4 mmol/L)的褪黑素干预人胃癌SGC-7901 细胞 24 h，应用划痕实验、Transwell小室法检测胃癌细胞迁移和侵袭能力的改变；ELISA试剂盒检测培养液上清中基质金属蛋白酶（MMP）-2、MMP-9的表达，Real-time PCR检测胃癌细胞MMP-2、MMP-9、细胞间黏附分子1(ICAM-1）和CD44基因表达；免疫印迹法检测ICAM-1、CD44、p-P38、P38和磷酸化丝裂原活化蛋白激酶激酶（p-MKK）3/6蛋白表达。 结果 褪黑素抑制胃癌细胞的迁移和侵袭，并呈剂量依赖性。与空白对照组比较，褪黑素降低胃癌细胞基质金属蛋白酶（MMP)-2、MMP-9和ICAM-1、CD44的表达，并抑制p-P38、P38和p-MKK3/6的表达。 结论 褪黑素通过抑制胃癌细胞MMP-2、MMP-9、ICAM-1和CD44的表达从而抑制胃癌细胞的迁移和侵袭能力，抑制作用可能与p38MAPK信号通路有关。

Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901

Objective To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin (0.1, 1, 2 and 4 mmol/L) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase(MMP)-2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9, intercellular cell adhesion molecule-1(ICAM-1) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase(p-MKK)3/6 were detected by Western blotting. Results Melatonin inhibited the migration and invasion of gastric cancer cells in a dose-dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells. Conclusion Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway. 