| 利用亮甲酚蓝染色筛选优质猪卵母细胞 |
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| 引用本文： | 田江天,王振坤,郑重,孙爽,徐萌,刘忠华.利用亮甲酚蓝染色筛选优质猪卵母细胞[J].解剖学报,2009,40(2):340-343. |
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| 作者姓名： | 田江天 王振坤 郑重 孙爽 徐萌 刘忠华 |
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| 作者单位： | 1.东北农业大学生命科学学院，哈尔滨 150030;2.浙江大学材料与化学工程学院材料科学与工程学系，杭州 310027 |
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| 基金项目： | 黑龙江省自然科学基金，黑龙江省教育厅新世纪人才基金 |
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| 摘 要： | 目的 利用亮甲酚蓝(BCB)染色提高猪体外胚胎培养体系的效率.方法 对猪卵丘卵母细胞复合体(COCs)进行BCB染色,观察染色后猪卵母细胞成熟率和早期胚胎发育能力并进行分析. 结果 着色组(BCB+)猪卵母细胞达到减数分裂Ⅱ(MⅡ)期的比率(91.6%)显著高于未着色组(BCB-)(64.0%)和对照组猪卵母细胞(77.5%)(P<0.05);BCB+孤雌猪胚胎的囊胚率(34.9%)显著高于BCB-(9.5%)和对照组(23.1%)(P<0.05);BCB+核移植猪胚胎的囊胚率(23.0%)显著高于BCB-(5.0%)和对照组(14.1%)(P<0.05).结论 BCB染色是一种有效筛选具有较强发育能力猪卵母细胞的方法.
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| 关 键 词： | 卵母细胞 核移植 亮甲酚蓝（BCB）染色 猪 |
| 收稿时间： | 2007-12-19 |
| 修稿时间： | 2008-4-8 |
Selection of developmentally competent pig oocytes using brilliant cresyl blue staining |
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| TIAN Jiang-tian,WANG Zhen-kun,ZHENG Zhong,SUN Shuang,XU Meng,LIU Zhong-hua.Selection of developmentally competent pig oocytes using brilliant cresyl blue staining[J].Acta Anatomica Sinica,2009,40(2):340-343. |
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| Authors: | TIAN Jiang-tian WANG Zhen-kun ZHENG Zhong SUN Shuang XU Meng LIU Zhong-hua |
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| Institution: | 1.College of Life Science, Northeast Agricultural University, Haerbin 150030, China;2.Department of Materials Science and Engineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | P>Objective To enhance efficiency of EM>in vitro/EM> embryos culture system of pig using brilliant cresyl blue (BCB) stain. Methods To stain the cumulus oocyte complexes through BCB then evaluate oocytes maturation rates and developmental competence of early embryos. Results The percentage (91.6%) of the MⅡ stage oocytes of pigs was higher in the BCB colorable group (BCBSUP>+/SUP> ) than those (64.0%) in the non colorable (BCBSUP>－/SUP>) and control (77.5%) groups (EM>P/EM><0.05). The pig parthenogenetic blastocyst rate (34.9%) was significantly higher in the BCB+ group than those in the control and BCBSUP>－/SUP> groups, (23% and 9.5%) respectively (EM>P/EM><0.05). The rate (23.9%) of pig somatic cell nucleus-transferred embryos into blastocyst was significantly higher in the BCBSUP>+/SUP> group than those in the control and the BCB |
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| Keywords: | Oocyte Nuclear transfer Brilliant cresyl blue （BCB）staining Pig |
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