SARS患者脾内IL-6、IFN-β和IP-10的免疫组织化学观察及定量分析

目的 通过对严重急性呼吸综合征(SARS)患者脾内白细胞介素6(IL-6),干扰素β(IFN-β)和干扰素γ诱导蛋白10(IP-10)的免疫组织化学结果分析,深入探讨SARS患者脾组织的病理变化及其发病机制. 方法 通过免疫组织化学技术观察6例SARS死亡患者脾和6例意外死亡者正常脾组织细胞内IL-6、IFN-β和IP-10的表达情况,并进行图像分析.结果 SARS患者脾红髓内含大量IL-6阳性细胞,阳性产物呈团块状分布于细胞质和细胞核.图像分析结果显示,SARS患者脾红髓内IL-6阳性细胞的平均吸光度值(AA)与正常对照组比较,有显著性差异(P<0.05);SARS患者脾红髓内阳性细胞的细胞核、细胞质呈IFN-β阳性,阳性产物呈棕黄色团块状,图像分析结果显示,SARS患者脾组织内IFN-β阳性细胞的AA值与正常对照组比较,有显著性差异(P<0.05);SARS患者脾红髓内含大量IP-10阳性细胞,阳性产物呈棕黄色团块状,分布于细胞核和细胞质.图像分析结果显示,SARS患者脾组织内IP-10阳性细胞的AA值与正常对照组比较,有显著性差异(P<0.05).3种细胞因子在SARS患者残存的脾白髓内均呈阴性. 结论 SARS患者脾内细胞的IL-6、IFN-β和IP-10反应均相对增强,提示SARS患者脾组织的病理性损伤以及免疫功能缺陷可能与上述促炎症因子、免疫抑制因子及趋化因子的相对增多有关.

Immunohistochemical observation and quantitative analysis of IL-6, IFN-βand IP-10 in human spleens with severe acute respiratory syndrome

Objective To investigate the pathological changes and pathogeneses of spleen in severe acute respiratory syndrome(SARS) with immunohistochemical observation and quantitative analysis of the expressions of interleukin 6 (IL-6)，interferon β (IFN-β) and IFN-γ inducible protein 10 (IP-10). Methods Three specific antibodies were used to detect the expression of IL-6，IFN-β and IP-10 in the spleens of six cases who died of SARS and six normal cases as the controls with immunohistochemistry. The results were analyzed with image analysis system. Results In the splenic red pulp of SARS patients, lots of cells expressed IL-6 averagely, as bolus in cytoplasm and nucleus, and compared with normal cases, average absorbance (AEM>A/EM>) in IL-6 positive cells had significant difference (EM>P/EM><0.05). The IFN-β positive cells dispersed in splenic red pulp of SARS patients, yellow bolus was in cytoplasm and nucleus, compared with normal cases, AEM>A/EM> in IFN-β positive cells had significant difference (EM>P/EM><0.05). There were lots of IP-10 positive cells in splenic red pulp of SARS patients, the positive productions as bolus were in cytoplasm and nucleus, and compared with normal cases, AEM>A/EM> in IP-10 positive cells had significant difference (EM>P/EM><0.05). In the relic splenic corpuscle and periarterial lymphatic sheath, immunostainings of these three cytokines were all negative. Conclusion Immunohistochemical intensities of IL-6, IFN-β and IP-10 in spleen of SARS patient