Expression of integrin subunits correlates with differentiation of epithelial cell lineages in developing human gastric mucosa |
| |
Authors: | Chénard M Basque J R Chailler P Tremblay E Beaulieu J F Ménard D |
| |
Institution: | (1) MRC Group on the Functional Development and Physiopathology of the Digestive Tract, Department of Anatomy and Cell Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4 e-mail: dmenard@courrier.usherb.ca Tel.: +1 819-564-5278, Fax: +1 819-564-5320, CA |
| |
Abstract: | Laminins may play important roles in gastric gland development due to the differential localization of their α chains in human
fetal fundic (oxyntic) mucosa. To extend this hypothesis, the current investigation was undertaken to compare the anatomical
and cellular distribution of epithelial integrin subunits with those of laminin α chains in the human stomach at different
ages (8–22 weeks of gestation) using indirect immunofluorescence. In the body, fundus and antrum regions, the β1 and α6 subunits
were associated with the entire epithelium at all developmental stages in the same way as laminin chains (α1/α5) detected
with 4C7 monoclonal antibody. By contrast, the α3 and β4 subunits of α3β1 and α6β4 integrins together with the laminin α3-chain
were concentrated in the surface and foveolus compartments composed of differentiating mucous cells. Most importantly, the
α2 integrin subunit was expressed in a rather complex pattern: (1) it was located at the base and at cell-cell boundaries
of surface/foveolar epithelium, (2) was specifically repressed in differentiating parietal cells, and (3) its expression increased
in maturing glands, where it became concentrated at the basal pole of epithelial cells simultaneously exhibiting a strong
reactivity for laminin-2 (α2-chain). Taken altogether, our observations provide new evidence for the implication of different
laminins and their receptors in the development of all human gastric epithelial lineages, surface mucous cells or glandular
cells. The coordinated expression of α2 and α3 integrin subunits as well as the cellular re-distribution of α2β1 integrin
likely represent key events for the differentiation of glandular secretory cell types, especially maturing chief cells responsible
for the synthesis/secretion of gastric digestive enzymes in fundic-type glands.
Accepted: 26 March 2000 |
| |
Keywords: | Stomach Gastric glands Cell-matrix interactions Gland morphogenesis Human fetus |
本文献已被 PubMed SpringerLink 等数据库收录! |
|