小鼠心脏发育过程中Cx43表达的时空变化

目的：探讨小鼠心脏发育过程中连接蛋白43（connexin43，Cx43）表达的时空变化。方法：应用SP免疫组化和图像分析方法，观测胎小鼠、出生1d、6d、2月和14月小鼠心肌细胞Cx43的表达。结果：1、胎小鼠和出生1d小鼠心肌细胞Cx43呈斑点状遍布于心肌细胞侧-侧连接和细胞质内，闰盘处极少。2、出生6d时Cx43开始向端闰盘处聚集，2月和14月小鼠心室肌细胞Cx43已经典型分布于闰盘处，而心房仍分布于细胞侧面连接处和细胞质。3、分布密度总规律是：胎小鼠〈出生1d〈6d〉2月〉14月。结论：小鼠心肌细胞Cx43分布随心脏发育逐渐向闰盘处重排，其密度逐渐下降。心房肌Cx43的表达与分布具有一定的幼稚性。

The Space- time Changes of Cx43 Expression During the Development Process of Mouse Myocardium

Objective:To investigate the space-time changes of Connexin 43(Cx43)expression during development process of mouse myocardium.Methods:The Cx43 expressions on cardiac muscle cells in mice at fetus and postnatal 1 day,6 days,2 months and 14 months were determined by immunohistochemistry and image analysis.Results:1. The Cx43 immuno-labelling present punctate distribution in cytoplasm and junction of both neighboring cadiocytes,and few located at intercalated disk in fetus and postnatal one day mice.2.The Cx43 immuno-labelling prone to assembling to intercalated disk in postnatal 6 days mice.Cx43 positive granules distributed typically confined to the site of intercalated disk in ventricular myocytes and irregularly distributed in cellular lateral surfaces and cytoplasm of auricular myocytes in postnatal 2 and 14 months mice.3.The general tendency of Cx43 distributed density was as follow:fetus < postnatal one days<6 days>2 months>14 months.Conclusion:The Cx43 rearrangements were confined to the intercalated disk following the heart development of mice, with gradually decreased density. The Cx43 expression and distribution in auricular myocytes have some immature.