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Are thrombocyte membranes altered in Alzheimer's disease? A morphometric and biochemical study |
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| Authors: | GJCGM Bosman JH Schuurmans Stekhoven JJ Melenhorst AJ Van Zuylen IGP Bartholomeus PJC Van Kalmthout WJ De Grip |
| Institution: | a Department of Biochemistry, The Netherlands b Institute of Pathology, Department of Electronmicroscopy, Faculty of Medicine, University of Nijmegen, The Netherlands c Psychogeriatric Nursing Home “St. Joachim en Anna”, Nijmegen, The Netherlands |
| Abstract: | Morphometric analysis of thrombocytes from patients with Alzheimer's disease, from patients with multi-infarct dementia, and from young and agematched healthy control donors, did not reveal any Alzheimer-related increase in internal membranes. Biochemical analysis showed a reduced cholesterol content of thrombocyte membrane preparations from Alzheimer patients relative to age-matched controls, but not relative to multi-infarct dementia patients. Overall distribution of protein kinase C activity (PKC) between cytosol and membrane, in resting as well as in activated thrombocytes from Alzheimer patients, was similar to that in the control groups. However, both Alzheimer and multi-infarct dementia patients had lower cytosolic levels of basal kinase and PKC activities than age-matched controls, while only Alzheimer patients had lower cytoskeletal PKC activity than controls. |
| Keywords: | Alzheimer's disease Dementia Multi-infarct dementia Thrombocyte Membrane Kinase Protein kinase C Cholesterol Fatty acids Electron microscopy Morphometry |
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