| 野外采集标本中人埃立克体16S rRNA基因扩增方法及应用 |
| |
| 引用本文： | 高东旗,曹务春,张习坦,赵秋敏,童贻刚.野外采集标本中人埃立克体16S rRNA基因扩增方法及应用[J].寄生虫与医学昆虫学报,2001,8(3):175-180. |
| |
| 作者姓名： | 高东旗 曹务春 张习坦 赵秋敏 童贻刚 |
| |
| 作者单位： | 军事医学科学院微生物流行病研究所, |
| |
| 基金项目： | 国家自然科学基金资助,批准号:39970653 |
| |
| 摘 要： | 本文应用特异引物,通过多对半巢式PCR从采自内蒙古大兴安岭的蜱标本中扩增出人粒细胞埃立克体和查菲埃立克体的16SrRNA全基因,一方面提供了这2种埃立克体存在于中国的证据,另一方面为今后进行埃立克体病的病原、媒介和宿主的调查研究提供了一个可行的方法.并从一只大林姬鼠的脏器中检测到EC16SrRNA基因片段.
|
| 关 键 词： | 人粒细胞埃立克体 查菲埃立克体 16S rRNA基因 半巢式PCR |
| 修稿时间： | 2000年10月9日 |
THE AMPLIFYING METHODS AND APPLICATION OF THE 16S rRNA GENES OF TWO KINDS OF
HUMAN EHRLICHIA SPECIES |
| |
| Gao Dongqi Cao Wuchun Zhang Xitan Zhao Qiumin Tong Yigang Institute of Military Medicine,Beijing Military Region,Shijiazhuang.THE AMPLIFYING METHODS AND APPLICATION OF THE 16S rRNA GENES OF TWO KINDS OF
HUMAN EHRLICHIA SPECIES[J].Acta Parasitologica et Medica Entomologica Sinica,2001,8(3):175-180. |
| |
| Authors: | Gao Dongqi Cao Wuchun Zhang Xitan Zhao Qiumin Tong Yigang Institute of Military Medicine Beijing Military Region Shijiazhuang |
| |
| Institution: | Gao Dongqi Cao Wuchun Zhang Xitan Zhao Qiumin Tong Yigang * Institute of Military Medicine,Beijing Military Region,Shijiazhuang 050081 |
| |
| Abstract: | The entire 16S rRNA genes of human granulocytic ehrlichia and Ehrlichia chaffeensis were amplified using some specific primers by semi nested PCR in ticks collected from Daxingan Mountains,Inner Mongolia. The findings offered the evidence that the two agents exist in China,and also provided feasible methods for searching pathogens,vectors and animal hosts of human ehrlichioses. In addition, the EC 16S rRNA gene sequence was detected from the internal organ(liver and spleen) of a wild mice (Apodemus peninsulae) collected from the woods of the area. |
| |
| Keywords: | Human granulocytic ehrlichia Ehrlichia chaffeensis16S rRNA gene Semi PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
