BPX-80离心式血泵中剪切力引发血管性血友病因子机械损伤的评价

目的 对BPX-80离心式血泵中剪切力引起血管性血友病因子（von Willebrand Factor，vWF）的损伤进行评价，判断其是否可以作为vWF损伤研究的参照泵。方法 按照ASTM标准搭建体外溶血测试平台，使用新鲜猪血在此平台对BPX-80离心式血泵进行8 h测试，然后评估每小时血液样本的溶血水平和vWF损伤，并与静态对照组进行比较。结果 在整个实验过程中，BPX-80的溶血指数保持稳定且较低；高分子量vWF多聚体有少量降解，与静置对照组相比无显著性差异；vWF抗原含量无明显变化，与静置对照组趋势基本一致。结论 BPX-80离心式血泵血液相容性良好，可以作为vWF损伤以及溶血评价实验的基准参照泵，从而为新型血泵的设计与优化提供参考。

Evaluation on Mechanical Damage of vWF Induced by Shear Stress in BPX-80 Centrifugal Blood Pump

Objective To evaluate the damage of von Willebrand factor (vWF) induced by shear stress in BPX-80 centrifugal blood pump, and determine whether it can be used as a reference pump for vWF damage research. Methods An in vitro hemolysis test platform was established according to the ASTM standards. The BPX-80 centrifugal blood pump was tested for 8 hours by using fresh porcine blood. The hemolysis level and vWF damage of hourly blood samples were then evaluated and compared with the static control group. ResultsThe hemolysis index of BPX-80 was stable and low during the test; vWF polymer with high molecular weight had a small amount of degradation, and showed no significant difference compared with the static control group; there was no significant change in the concentration of vWF antigen, which was basically consistent with the trend of the static control group. Conclusions BPX-80 centrifugal blood pump has good blood compatibility and can be used as the reference pump for vWF damage and hemolysis evaluation, thereby providing guidance for the design and optimization of new blood pumps.