硫化氢在雌激素促血管内皮细胞增殖中的作用研究

目的研究硫化氢（H2S）在雌激素促进血管内皮细胞增殖中的作用。方法利用培养的脐静脉血管内皮细胞，以雌激素处理后，Western blot检测H2S产生关键酶CSE（胱硫醚-γ-裂解酶）的蛋白表达情况；亚甲基蓝法测定血管内皮细胞释放H2S含量；MTT法检测血管内皮细胞增殖情况。结果不同浓度（1nmo1／L～1μmol／L）的雌激素处理血管内皮细胞48h后．均可促进CSE蛋白表达和内皮H2S释放。不同浓度的雌激素均能明显促进血管内皮细胞增殖。以CSE特异性抑制剂PPG抑制H2S产生后，雌激素促进血管内皮增殖的能力受到显著抑制。结论雌激素可通过促进CSE蛋白表达促进血管内皮细胞释放H2S，进而促进血管内皮细胞增殖。

Estrogen promotes vascular endothelial cell proliferation via hydrogen sulfide

Objective To investigate the role of hydrogen sulfide （H2S） in estrogen-induced vascular endothelial cell proliferation. Methods Human umbilical vein endothelial cells were cultured and treated with 1713-estradiol （E2）. The expression of cystathionine-γ-lyase （CSE） was examined by western blot method. Methylene blue assay was used to determine the level of H2S. Cell proliferation was calculated by 3- （4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTF） assay. Results Treatment with different doses of E2 （ 1 nmol/L-1 μmol/L） for 48 h all resulted in increased expression of CSE protein and H2S release. Meanwhile, E2 （ 1 nM- 1 μM） promoted vascular endothelial cell proliferation, which was inhibited by the addition of specific CSE inhibitor PPG. Conclusions E2 enhances CSE protein expression and the release of H2S, thus promotes vascular endothelial cell proliferation.